The effect of N-stearoylethanolamine on cholesterol content, fatty acid composition and protein carbonylation level in rats with alimentary obesity-induced insulin resistance.

The effect of N-stearoylethanolamine (NSE) on liver free fatty acid composition, cholesterol content and carbonylated protein level in rats with obesity-induced insulin resistance (IR) was studied in the work. The experimental insulin resistance was induced by prolonged high fat diet (58% of energy derived from fat) for 6 months combined with one injection of low-dose (15 mg/kg) of streptozotocin. The lipid assay showed a rise in liver free cholesterol content anda significant reduction in cholesterol esters level.

[Cardioprotective effect of N-stearoylethanolamine under the anaphylactic shock in guinea pigs].

It was shown for the first time that endogenous cannabimimetic compound N-stearoylethanolamine (NSE) administered at the dose 65 mg/kg of body weight during two weeks before anaphylaxis had cardioprotective properties on the model of anaphylactic shock in the guinea pigs. NSE reliably normalized the content of histamine and nitric oxide, decreased the activity both inducible and constitutive NO-synthases, prevented accumulation of thiobarbituric acid reactive substances (TBARS) and normalized the activity of catalase, superoxide dismutase and glutathione peroxidase in cardiomyocytes of animals under anaphylaxis. About of 70% of the NSE-treated animals survived under the anaphylactic shock.

[Structural features of immunoglobulin G from leukemic cattle].

Structural peculiarities of immunoglobulin G (IgG) in cattle with leukemia were studied using the method of dansyl-finger prints and proteolytic fragmentation with pepsin. It is stated that the protein from the blood of leukemic animals differs from the similar IgG subfraction of healthy animals in the amount of peptides: their number is 39 in sick animals, 41--in healthy ones. The studied protein is splitted into three fragments under the effect of pepsin.

[Carbohydrate component of immunoglobulin G in cattle suffering from leukosis].

No essential differences are found in the composition and total amount of carbohydrates in the studied preparations of the immunoglobulin G subfraction in cattle suffering from leucosis and of the immunoglobulin G subfraction, identical in evolution, in healthy animals. It is shown that the main mass of carbohydrates is connected with Fc-fragment and heavy chains of the protein under study.

[Physicochemical properties and amino acid composition of papain fragments of immunoglobulin G isolated from blood of cows suffering from leukosis].

It is shown that the peculiar to the malignant growth protein isolated from blood of cattle suffering from leukosis is more stable to the effect of papain than the human protein characteristic of the malignant growth. Papain breaks the studied protein up into three components, the sedimentation constants of Fab- and Fc-fragments isolated from the papain hydrolysate are 3,9 and 3,6S and their molecular mass --55000 and 45000 Daltons, respectively. In the Fab-fragment the contents of lysine, glutaminic acid, leucine, serine are higher and those of arginine, alanine, valine, methionine, phenylalanine and isoleucine are lower as compared with the Fab-fragment isolated from immunoglobulin G of healthy animals.