Publications by authors named "E E Beketov"

Article Synopsis
  • The study compared type I collagen and GelMA hydrogels for their effectiveness in forming hyaline cartilage when implanted in rats.
  • Chondrocytes were extracted from newborn rats and cultured, then implanted into two groups of Wistar rats using both types of hydrogels.
  • Both hydrogels were successful in forming cartilage tissue after 26 days, showing a mixed chondrocyte phenotype, but further research is needed to understand the mechanisms of cartilage formation for each hydrogel.
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Gelatin methacryloyl (GelMA) has recently attracted increasing attention. Unlike other hydrogels, it allows for the adjustment of the mechanical properties using such factors as degree of functionalization, concentration, and photocrosslinking parameters. In this study, GelMA with a high degree of substitution (82.

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The aim of this study was to verify the applicability of high-concentration collagen-based bioink with MSC (ADSC) and decellularized ECM granules for the formation of cartilage tissue de novo after subcutaneous implantation of the scaffolds in rats. The printability of the bioink (4% collagen, 2.5% decellularized ECM granules, derived via 280 μm sieve) was shown.

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In recent years, decellularized tissues have evolved into a new, full-fledged platform for the creation of tissue-engineered constructions. Extracellular matrix (ECM) of each tissue provides a unique tissue-specific microenvironment for resident cells with the structure and biochemical signaling required for their functioning. The decellularized ECM (dECM) has been established to influence cell differentiation.

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The study was aimed at the applicability of a bioink based on 4% collagen and chondrocytes for cartilage formation. Extrusion-based bioprinting was used for the biofabrication. The printing parameters were tuned to obtain stable material flow.

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