Publications by authors named "E E Baetge"
The Scientific Challenge of Expanding the Frontiers of Nutrition.
Nestle Nutr Inst Workshop Ser
October 2016
Nutritional research is entering a paradigm shift which necessitates the modeling of complex interactions between diet, genetics, lifestyle, and environmental factors. This requires the development of analytical and processing capabilities for multiple data and information sources to be able to improve targeted and personalized nutritional approaches for the maintenance of health. Ideally, such knowledge will be employed to underpin the development of concepts that combine consumer and medical nutrition with diagnostic targeting for early intervention designed to maintain proper metabolic homeostasis and delay the onset of chronic diseases.
View Article and Find Full Text PDFType 1 diabetes (T1D) is an autoimmune disease caused by T cell-mediated destruction of insulin-producing β cells in the islets of Langerhans. In most cases, reversal of disease would require strategies combining islet cell replacement with immunotherapy that are currently available only for the most severely affected patients. Here, we demonstrate that immunotherapies that target T cell costimulatory pathways block the rejection of xenogeneic human embryonic-stem-cell-derived pancreatic endoderm (hESC-PE) in mice.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on developing a scalable and regulated manufacturing process for creating pancreatic progenitors from human embryonic stem cells (hESC) to treat type 1 diabetes.
- Researchers optimized methods for expanding and differentiating the CyT49 cell line, resulting in a resource that can produce functional insulin-secreting cells in a controlled environment.
- Upon implantation into mice, these cells formed mature pancreatic tissue that effectively managed glucose levels, indicating progress toward a potential clinical therapy for diabetes.
Article Synopsis
- Researchers utilized a flow cytometry-based method to identify specific cell-surface markers that help separate different pancreatic cell types derived from human embryonic stem cells.
- The study found that pancreatic endoderm cells could be enriched using the markers CD142, while endocrine cells were enriched using CD200 and CD318.
- When transplanted into mice, the enriched endoderm cells developed into various functional pancreatic cells, including insulin-producing cells, proving their potential as pancreatic progenitors, whereas the polyhormonal endocrine cells mainly generated glucagon cells.