Substituted benzopyranobenzothiazinones. Synthesis and estrogenic activity on MCF-7 breast carcinoma cells.

In the search for new agents with estrogenic activity mediated by estrogen receptors (ER), six 6,12-dihydro-1-benzopyrano[3,4-b][1,4]benzothiazin-6-ones 3a-f were synthesized. These compounds were readily prepared by the addition of 2-aminothiophenol 2 to substituted 4-hydroxycoumarin derivatives 1a-e. The estrogenic effect has been evaluated on the proliferation of MCF-7 breast adenocarcinoma cells and the specificity of described compounds was evaluated by the inhibition of their effect by ICI 182,780, an antiestrogenic compound.

[Reference values of plasma and lipoprotein cholesterol and triacylglycerols in healthy children].

A sample of a reference population was selected from an all-inclusive population by a method based on strict physiological and clinical criteria. Sociological and ethnic factors as well as the biological plasma evaluation of the parents were used as family exclusion parameters. Cholesterol and triacylglycerols were measured in total plasma as well as in each lipoprotein fraction isolated by ultracentrifugation.