Publications by authors named "E Darzynkiewicz"
All cells in our body are equipped with receptors to recognize pathogens and trigger a rapid defense response. As a result, foreign molecules are blocked, and cells are alerted to the danger. Among the many molecules produced in response to viral infection are interferon-induced proteins with tetratricopeptide repeats (IFITs).
View Article and Find Full Text PDFArticle Synopsis
- mRNA-based drugs are rapidly advancing, particularly as viral vaccines, with potential uses in various diseases due to their easy and adaptable production process.
- The structure of mRNA components, especially modifications at the 5' cap, significantly influences its effectiveness and longevity in medical applications.
- This study focuses on synthesizing modified cap analogues and testing these compounds for their ability to act as translation inhibitors and improve mRNA preparation.
Eukaryotic initiation factor 4E (eIF4E) is a pivotal protein involved in the regulatory mechanism for global protein synthesis in both physiological and pathological conditions. MicroRNAs (miRNAs) play a significant role in regulating gene expression by targeting mRNA. However, the ability of miRNAs to regulate eIF4E and its phosphorylation remains relatively unknown.
View Article and Find Full Text PDFArticle Synopsis
- Nudt15 is a well-studied enzyme from the NUDIX protein family, primarily for its effects on thiopurine drugs used in cancer and inflammatory disease treatment.
- Besides its known activity on thiopurines, Nudt15 also acts on various nucleotide substrates, and there's speculation about its role in the degradation of mRNA through hydrolysis of mGDP.
- Recent research showed that Nudt15 has moderate activity with methylated forms of GDP and GTP, suggesting these can inform the design of new small molecule inhibitors targeting Nudt15.
Mesenchymal stem cell engineering by ARCA analog-capped mRNA.
Anna Andrzejewska Renata Grzela Anna Stankiewicz-Drogon Piotr Rogujski Siranjeevi Nagaraj Edward Darzynkiewicz
Mol Ther Nucleic Acids
September 2023
Article Synopsis
- The study examines factors affecting mRNA engineering of mesenchymal stem cells (MSCs), focusing on transfection methods, mRNA purification, and capping types.
- Results show that Lipofectamine 2000 is superior to TransIT for MSC transfection, while HPLC purification is essential for maintaining MSC health.
- It concludes that using Lipofectamine 2000 with HPLC-purified mRNA and β-S-ARCA D1 capping achieves enhanced protein production, albeit with reduced MSC metabolic activity.