One-step route to tricyclic fused 1,2,3,4-tetrahydroisoquinoline systems via the Castagnoli-Cushman protocol.

The Castagnoli-Cushman reaction of 3,4-dihydroisoquinolines with glutaric anhydride, its oxygen and sulfur analogues was investigated as a one-step approach to the benzo[]quinolizidine system and its heterocyclic analogs. An extension towards the pyrrolo[2,1-]isoquinoline system was achieved with the use of succinic anhydride. The results are evidence of an unexplored method for the access of the aforementioned tricyclic annelated systems incorporating a bridgehead nitrogen atom.

Natural and synthetic cage compounds incorporating the 2,9,10-trioxatricyclo[4.3.1.03,8]decane type moiety.

The 2,9,10-trioxatricyclo[4.3.1.

[Evaluation of the biological activity of allergens by different in vivo and in vitro immunological methods].

The specific activity of different allergens has been studied in vivo and in vitro by certain immunological methods (complement inactivation, mastocyte degranulation, neutrophil damage index, lymphocyte blast transformation). The results obtained by the skin allergic tests have been found to correlate well with those obtained by the in vitro methods. This allows one to use these methods for a more complete characterization of allergens and their standardization.