The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods.

The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, e.

Transcriptional Profiling of Filovirus-Infected Nonhuman Primate Samples.

Transcriptional analysis is a powerful tool to help decipher the host response to filovirus infections. In this chapter, we describe how transcriptional analysis can be performed on samples obtained from nonhuman primates infected with filoviruses, specifically Marburg virus, for assessment of the RNA changes over the course of the infection and disease.

The IBEX Knowledge-Base: Achieving more together with open science.

Iterative Bleaching Extends multipleXity (IBEX) is a versatile method for highly multiplexed imaging of diverse tissues. Based on open science principles, we created the IBEX Knowledge-Base, a resource for reagents, protocols and more, to empower innovation.

Rebalancing Viral and Immune Damage versus Tissue Repair Prevents Death from Lethal Influenza Infection.

Maintaining tissue function while eliminating infected cells is fundamental to host defense. Innate inflammatory damage contributes to lethal influenza and COVID-19, yet other than steroids, immunomodulatory drugs have modest effects. Among more than 50 immunomodulatory regimes tested in mouse lethal influenza infection, only the previously reported early depletion of neutrophils showed efficacy, suggesting that the infected host passes an early tipping point in which limiting innate immune damage alone cannot rescue physiological function.

Causality Bounds on Dissipative General-Relativistic Magnetohydrodynamics.

We derive necessary and sufficient conditions under which a large class of relativistic generalizations of Braginskii's magnetohydrodynamics with shear, bulk, and heat diffusion effects is causal and strongly hyperbolic in the fully nonlinear regime in curved spacetime. We find that causality severely constrains the size of nonideal effects and the onset of kinetic instabilities. Our results are crucial for assessing the regime of validity of fluid dynamical simulations of plasmas near supermassive black holes.