Systematic identification of gene combinations to target in innate immune cells to enhance T cell activation.

Genetic engineering of immune cells has opened new avenues for improving their functionality but it remains a challenge to pinpoint which genes or combination of genes are the most beneficial to target. Here, we conduct High Multiplicity of Perturbations and Cellular Indexing of Transcriptomes and Epitopes (HMPCITE-seq) to find combinations of genes whose joint targeting improves antigen-presenting cell activity and enhances their ability to activate T cells. Specifically, we perform two genome-wide CRISPR screens in bone marrow dendritic cells and identify negative regulators of CD86, that participate in the co-stimulation programs, including Chd4, Stat5b, Egr2, Med12, and positive regulators of PD-L1, that participate in the co-inhibitory programs, including Sptlc2, Nckap1l, and Pi4kb.

Long-range potentials and dipole moments of the CO electronic states converging to the ground dissociation limit.

The asymptotic, R → ∞, behavior of the potential-energy and dipole-moment functions (PEFs and DMFs) for all six (1,2)Σ+, (1,2)Π, Σ-, and Δ electronic states converging to the ground C(3P) + O(3P) dissociation limit of the CO molecule are studied in the framework of long-range (LR) perturbation theory. The analytical expressions for the leading coefficients of the LR expansion, C5/R5 for PEF and d4/R4 for DMF, in terms of the atomic quadrupole constants and static dipole polarizabilities are derived. The exact relationships between the LR coefficients for the states of different spatial symmetry are established as well.

Intensity anomalies in the rotational and ro-vibrational spectra of diatomic molecules.

We study the anomalies in the distributions of intensities of transitions in the purely rotational bands and the rotational branches of the vibrational bands within the unperturbed ground electronic states in spectra of diatomic molecules. While normally these distributions follow smooth patterns, sudden drops in intensity values are often observed. We analyze the origin of these anomalies in HF, DF, and CO and find that they are predominantly associated with specific forms of the dipole-moment functions (DMFs).

Peculiarities of high-overtone transition probabilities in carbon monoxide revealed by high-precision calculation.

In the recent work devoted to the calculation of the rovibrational line list of the CO molecule [G. Li et al., Astrophys.

Mechanisms of generation of local ΔpH in mitochondria and bacteria.

The concepts of global and local coupling between proton generators, the enzymes of the respiratory chain, and the consumer, the ATP synthase, coexist in the theory of oxidative phosphorylation. Global coupling is trivial proton transport via the aqueous medium, whereas local coupling implies that the protons pumped are consumed before they escape to the bulk phase. In this work, the conditions for the occurrence of local coupling are explored.