Publications by authors named "E Combe"
Hepatitis B virus (HBV) infection remains a significant global health challenge, leading to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC). Covalently closed circular DNA (cccDNA) and integrated HBV DNA are pivotal in maintaining viral persistence. Recent advances in CRISPR/Cas technology offer innovative strategies to inhibit HBV by directly targeting both cccDNA and integrated HBV DNA or indirectly by degrading HBV RNAs or targeting host proteins.
View Article and Find Full Text PDFArticle Synopsis
- The liver contains diverse populations of monocytes and macrophages, including liver-resident macrophages called Kupffer cells (KCs).
- This text outlines a protocol for isolating KCs from human liver tissue removed during surgeries, detailing steps for tissue dissociation and cell separation.
- It also includes instructions for validating the isolation process through immunofluorescence to confirm the presence of KCs by detecting the CD163 marker.
Chronic hepatitis B virus (HBV) infection remains a global health problem due to the lack of treatments that prevent viral rebound from HBV covalently closed circular (ccc)DNA. In addition, HBV DNA integrates in the human genome, serving as a source of hepatitis B surface antigen (HBsAg) expression, which impairs anti-HBV immune responses. Cytosine base editors (CBEs) enable precise conversion of a cytosine into a thymine within DNA.
View Article and Find Full Text PDFBackground: Endoscopic sleeve gastroplasty (ESG) is a minimally invasive procedure that has been demonstrated in the MERIT randomised, controlled trial to result in substantial and durable additional weight loss in adults with obesity compared with lifestyle modification (LM) alone. We sought to conduct the first cost-effectiveness analysis of ESG versus LM alone in adults with class II obesity (BMI 35.0-39.
View Article and Find Full Text PDF