Inhibition of forward and reverse transport of Ca via Na/Ca exchangers (NCX) prevents sperm capacitation.

Background: While calcium is known to play a crucial role in mammalian sperm physiology, how it flows in and out of the male gamete is not completely understood. Herein, we investigated the involvement of Na/Ca exchangers (NCX) in mammalian sperm capacitation. Using the pig as an animal model, we first confirmed the presence of NCX1 and NCX2 isoforms in the sperm midpiece.

Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity.

Background: The analysis of chromatin integrity has become an important determinant of sperm quality. In frozen-thawed bovine sperm, neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well understood. The aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics, and to assess if this parameter can predict pregnancy rates in cattle.

From secondary school to university: associations between sport participation and total and domain-specific sedentary behaviours in Spanish students.

Effective ways to reduce sedentary behaviour in adolescents are needed to mitigate the risk of chronic disease and poor mental health. Organised sport participation is the most practiced physical activity during adolescence. However, the influence sport participation has on sedentary behaviours remains unclear.