Leucocyte-migration-inhibition test in patients with colorectal cancer: clinicopathological correlations.

Leucocyte-migration-inhibition test was used to study the immune reactions of leucocytes from 136 colorectal cancer patients, 43 patients with non-cancerous chronic colorectal diseases and 82 controls, with saline extracts of HT29 line. A positive inhibition was found in only 43% of colorectal cancer patients. It was higher in carcinomas of limited extension than in invasive ones (64% against 39%).

Control of cell multiplication: preparation and immunological comparison of inhibitory factors in the homogenate and perfusate of the adult rat liver.

The control of organ cell multiplication by a negative feed-back mechanism implies that factors of the inhibitory mechanism are present in the blood and are produced by the target organ itself. We have examined the inhibitors which are present in the cytosol of adult rat liver as well as in efferent fluid from an isolated perfused organ. These two materials yielded predominantly protein molecules having in common a molecular weight of approximately 80 000 daltons and an isoelectric point between pH 8 and 9.

Use of a permanent cell line extracts to study the tumor associated immune reactions in colorectal cancer patients by leucocyte migration inhibition test.

Crude extracts of colorectal surgical tumors, either individual or pooled, and HT29 line cells were compared in leucocyte migration inhibition test. HT29 cell extract gave more positive reactions with leucocytes of colorectal cancer patients than the other ones, and less with control leucocytes. It was thus used for all the subsequent experiments.

[Relationship between the growth rate of the liver of young rats and the inhibitory effect of extracts of their liver on the multiplication of cultured cells].

We have shown that extracts of liver from young Rats are less active, than extracts of liver from adult Rats, in inhibiting the multiplication of cells in culture. This inhibitory activity is at a minimum in livers taken from 10 to 15 days old Rats, which corresponds to the time of maximum increase in weight of the liver. The existence of an inverse relationship between the inhibitory activity of these extracts and the state of proliferation of the liver suggests that the inhibitory substance contained in the liver extracts may act as a regulator of growth of the organ.