Publications by authors named "E Chaloupkova"

Article Synopsis
  • Tailocins are tiny protein complexes that act like phage tails and can inhibit closely related bacterial species, with R-type being common in Gammaproteobacteria and F-type being rare.
  • This study focused on a freshwater isolate, Pragia fontium 24613, which produces both R- and F-type tailocins, analyzing their ability to inhibit clinically relevant bacteria and identifying the related gene cluster.
  • The results showed that disrupting a specific protein affected R-tailocin production, and a comparison of genes revealed R-type tailocins are widely conserved among certain bacteria, while F-type tailocins show less conservation and presence, highlighting their antimicrobial potential against important pathogens.
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is one of the few species that belongs to the group of atypical hydrogen sulfide-producing enterobacteria. Unlike other members of this closely related group, is not associated with any known host and has been reported as a free-living bacterium. Whole genome sequencing and metabolic fingerprinting confirmed the phylogenetic position of inside the group of atypical HS producers.

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The complete genome sequence of Pragia fontium 24613 was determined using PacBio RSII, Roche 454, and SOLiD sequencing. A total of 3,579 genes were predicted, including 3,338 protein-coding sequences and 146 pseudogenes. This is the first whole-genome sequence of a strain belonging to the environmental genera of the family Enterobacteriaceae.

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The strain Escherichia coli Nissle 1917 (EcN) is widely used as an efficient probiotic in therapy and prevention of human infectious diseases, especially of the intestinal system. Concurrently, small adult pigs are being used as experimental omnivore models to study human gastrointestinal functions. EcN bacteria were applied to 6 adult healthy female pigs in a 2-week trial.

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