High-performance liquid chromatographic determination of vincristine sulfate in preformulation studies.

A fast and simple procedure was developed for the quantitative determination of vincristine sulfate for use in preformulation studies. The procedure involves the use of high-performance liquid chromatography with a reverse-phase column and a mobile phase containing the sodium salt of 1-pentanesulfonic acid for ion-pairing. The procedure has been shown to be specific for vincristine sulfate in the presence of forced degradation products of this substance, vinblastine (a structurally similar Vinca alkaloid), and several possible formula excipients.

The objective and timing of drug disposition studies, appendix IV. Phenylbutazone formulations: in vitro dissolution and in vivo performance.

Human plasma level studies were conducted on various phenylbutazone formulations. The resultant data indicated that the nature of the formulation had a marked influence on the rate of phenylbutazone absorption. An in vitro dissolution rate apparatus was employed in an attempt to predict potential absorption of phenylbutazone from a given formulation.