Determination of delta 9-tetrahydrocannabinol levels in brain tissue using high-performance liquid chromatography with electrochemical detection.

delta 9-Tetrahydrocannabinol (delta 9-THC) is the major psychoactive component of cannabis. To assist in investigating the mechanism(s) of action of delta 9-THC, a convenient method for determining its levels in brain tissue is required. We now describe a method for determining nanogram quantities of delta 9-THC in rat brain tissue.