Publications by authors named "E Breard"
Article Synopsis
- - Bluetongue (BT) and Epizootic Hemorrhagic Disease (EHD) are important animal diseases impacting cattle and sheep, caused by viruses transmitted by biting midges, with recent outbreaks noted in France during the summer of 2023.
- - Genomic analysis revealed three viruses involved in the outbreaks: EHDV-8, which matches strains found in southern Europe since 2021, and two different strains of BTV, including a novel BTV-8 strain and an established BTV-4 strain.
- - The 2023 outbreaks indicate unusual dynamics in the circulation and emergence of these viruses in France, prompting further investigation into the epidemiological factors contributing to their spread.
Article Synopsis
- Viral diseases can have different clinical outcomes based on the virus strain and individual host responses; understanding these differences is crucial for developing therapies and prognostic markers.
- The study focused on the bluetongue virus in sheep, exploring how variations in the virus lead to a range of clinical symptoms, from mild to severe disease.
- Researchers used machine learning to analyze 332 parameters, identifying five key processes (virus replication, immune response modulation, inflammation, vascular damage, and immunosuppression) that influence the severity of bluetongue infections.
The emergence of EHDV in Europe during the autumn of 2022 reinforces the need for molecular tools (RT-PCR) for rapid detection of animals infected with this virus. Viral genome testing can be performed on whole blood under anticoagulant, spleen, and bloody organ homogenates from ruminants. It can also be performed on cell culture following viral isolation tests.
View Article and Find Full Text PDFReal-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus.
Methods Mol Biol
August 2024
Real-time RT-PCR for the detection of epizootic hemorrhagic disease virus (EHDV) in clinical samples is a fast and sensitive tool for the diagnosis and confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification.
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