Pretreatment serum levels of C-reactive protein, alpha 1-antitrypsin, haptoglobin, alpha 1-acid glycoprotein and tissue polypeptide antigen in cervical carcinoma.

In order to evaluate the potentially additive information of some acute phase reactants to that provided by a general tumour marker, pretreatment concentrations of C-reactive protein, alpha 1-antitrypsin, haptoglobin, alpha 1-acid glycoprotein and tissue polypeptide antigen were determined in serum from healthy women, patients with dysplasia/or carcinoma in situ and patients with primary cervical carcinoma. Specificity varied from 95-100% and sensitivity from 16-29%. A correlation with clinical stage was found for all analytes except for alpha 1-antitrypsin.

Immunonephelometry of specific proteins in human seminal plasma.

Using immunonephelometry, we measured albumin, transferrin, alpha 1-antitrypsin, orosomucoid, hemopexin, IgG, IgA, and secretory IgA in seminal plasma of men who were attending a fertility clinic. Seminal plasma, obtained by centrifugation of semen, is a turbid liquid producing high blank values that were not diminished by ultracentrifugation, filtration, or treatment with protamine, dextran sulfate, or various lipidophilic gels. Pretreatment of seminal plasma with polyethylene glycol 6000 (final concentration, 40 g/L) considerably decreased the blank values without appreciably changing the relative concentration of the eight proteins studied.