Raman microscopy allows to follow internalization, subcellular accumulation and fate of iron oxide nanoparticles in cells.

An important issue in the context of both potenial toxicity of iron oxide nanoparticles (IONP) and their medical applications is tracking of the internalization process of these nanomaterials into living cells, as well as their localization and fate within them. The typical methods used for this purpose are transmission electron microscopy, confocal fluorescence microscopy as well as light-scattering techniques including dark-field microscopy and flow cytometry. All the techniques mentioned have their advantages and disadvantages.

Mechanisms of mitotic inhibition in human aorta endothelial cells: Molecular and morphological in vitro spectroscopic studies.

Mitotic inhibitors are drugs commonly used in chemotherapy, but their nonspecific and indiscriminate distribution throughout the body after intravenous administration can lead to serious side effects, particularly on the cardiovascular system. In this context, our investigation into the mechanism of the cytotoxic effects on endothelial cells of mitotic inhibitors widely used in cancer treatment, such as paclitaxel (also known as Taxol) and Vinca alkaloids, holds significant practical implications. Understanding these mechanisms can lead to more targeted and less harmful cancer treatments.

Publishing negative results is good for science.

Scientists face challenges in publishing negative results, because most scientific journals are biassed in accepting positive and novel findings. Despite their importance, negative results often go unpublished, leading to duplication of efforts, biassed meta-analyses, and ethical concerns regarding animal and human studies. In this light, the initiative by to collect and publish negative results in the field of microbiology is a very important and valuable contribution towards unbiassed science.