Characterization of cannabis strain-plant-derived extracellular vesicles as potential biomarkers.

The scientific interest in cannabis plants' beneficial properties has recently sparked certain interest in the possible functional characterization of plant-derived extracellular vesicles (PDEVs). Establishing the most appropriate and efficient isolation procedure for PDEVs remains a challenge due to vast differences in the physio-structural characteristics of different plants within the same genera and species. In this study, we employed a crude but standard isolation procedure for the extraction of apoplastic wash fluid (AWF) which is known to contain the PDEVs.

Prevention of physical restraint use among nursing home residents in Australia: The top three recommendations from experts and stakeholders.

Objective: To develop and prioritise recommendations to prevent the use of physical restraints among nursing home residents.

Methods: This study comprised two expert and stakeholder consultation forums using a modified nominal group technique and a follow-up electronic survey to rank the final set of recommendations.

Results: There were 15 recommendations formulated to prevent the use of physical restraint among nursing home residents.

The Nature and Extent of Physical Restraint-Related Deaths in Nursing Homes: A Systematic Review.

Objective: The aim of this study is to conduct a systematic review of the published research to examine the extent, nature, and risk factors of mortality due to physical restraint use.

Method: In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Statement, this review examined all peer-reviewed studies published in English describing mortality due to physical restraint in nursing home residents.

Results: The combined searches yielded 2,016 records of which eight articles were eligible for inclusion.

Physical restraint deaths in a 13-year national cohort of nursing home residents.

Objective: this paper aims to investigate the nature and extent of physical restraint deaths reported to Coroners in Australia over a 13-year period.

Methods: the study comprised a retrospective cohort study of residents dwelling in accredited nursing homes in Australia whose deaths were reported to the Coroners between 1 July 2000 and 30 June 2013 and was attributed to physical restraint.

Results: five deaths in nursing home residents due to physical restraint were reported in Australia over a 13-year period.

Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto.

Leptospira interrogans sensu stricto is responsible for the most frequent and severe cases of human leptospirosis. The epidemiology and clinical features of leptospirosis are usually associated with the serovars and serogroups of Leptospira. Because of the difficulties associated with serological identification of Leptospira strains, we evaluated a novel PCR-based method for typing L.