Exposure of Porcine Oocytes to Methylparaben During In Vitro Maturation Alters the Expression of Genes Involved in Cumulus Cell Expansion and Steroidogenesis, Decreasing Hyaluronic Acid and Progesterone Synthesis.

Parabens are widely used because of their antimicrobial properties in drugs, cosmetics, and food; however, it has been reported that methylparaben may adversely influence female reproduction. Methylparaben decreases oocyte in vitro maturation at a maturation inhibition concentration 50 of 780.31 μM but also decreases oocyte viability at a lethal concentration 50 of 2028.

Transfer RNA-derived fragment production in calves challenged with or co-infected with bovine viral diarrhea virus and in several tissues and blood.

Understanding the molecular mechanisms underlying immune response can allow informed decisions in drug or vaccine development, and aid in the identification of biomarkers to predict exposure or evaluate treatment efficacy. The objective of this study was to identify differentially expressed transfer RNA-derived fragments (tRFs) in calves challenged with () or co-infected with and bovine viral diarrhea virus (BVDV). Serum, white blood cells (WBC), liver, mesenteric lymph node (MLN), tracheal-bronchial lymph node (TBLN), spleen, and thymus were collected from Control ( = 2), (MB;  = 3), and co-infected (Dual; = 3) animals, and small RNAs extracted for sequencing.

Complete hybrid genome assembly of serotype A2 strain D95 isolated from ovine lung.

is a major bacterial pathogen associated with broncho- and fibrinous pneumonia in ruminants. Here, we report the complete genome sequence of an isolate of serotype A2 (D95) recovered from a pneumonic ovine lung. The D95 genome has a size of 2.

The effect of CGRP and SP and the cell signaling dialogue between sensory neurons and endothelial cells.

Increasing evidences demonstrate the role of sensory innervation in bone metabolism, remodeling and repair, however neurovascular coupling in bone is rarely studied. Using microfluidic devices as an indirect co-culture model to mimic in vitro the physiological scenario of innervation, our group demonstrated that sensory neurons (SNs) were able to regulate the extracellular matrix remodeling by endothelial cells (ECs), in particular through sensory neuropeptides, i.e.

Complete genome sequence of a strain 91 isolated from a beef calf with pneumonia.

is an important causative agent of bovine respiratory disease complex. Here, we report the complete genome sequence of a strain 91, which was isolated from a pneumonic lung tissue sample collected from a beef calf.