Publications by authors named "E BIERNAT"

This study aimed to examine potential changes in the anthropometric and motor characteristics of volleyball players aged 17.98 ± 0.51 years after participation in a week-long sports camp.

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Background: Many individuals who use the sauna at a temperature of 120°C of higher are not aware of the negative consequences of extreme thermal stress. Despite extensive research into sauna use, the impact of extreme thermal stress on the physiological and psychological characteristics of sauna users have not been examined to date.

Aim: The aim was to determine the effect of 20 min sauna sessions with a temperature of 80°C and 120°C on the physiological and psychological characteristics of women who sporadically visit the sauna.

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Introduction And Objective: Relief from routine physical tasks leads to increasingly sedentary behaviour (SB) - a risk factor for non-communicable chronic diseases. It is necessary to gather interdisciplinary knowledge about the possibilities of its reduction. The aim of the study is to analyse SB and its conditions among Polish white-collar and blue-collar workers.

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RSC (remodels the structure of chromatin) is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. RSC utilizes its ATPase subunit, Sth1, to slide or remove nucleosomes. RSC has been shown to regulate the width of the nucleosome-depleted regions (NDRs) by sliding the flanking nucleosomes away from NDRs.

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Article Synopsis
  • Nucleosomes are fundamental units of chromatin that can hinder various DNA processes, creating a need for regulatory mechanisms to manage DNA accessibility.
  • The mobilization and positioning of nucleosomes are influenced by chromatin remodeling complexes and can be analyzed using the technique of micrococcal nuclease (MNase) digestion combined with chromatin immunoprecipitation (ChIP) and sequencing.
  • The text provides a detailed methodology for conducting MNase digestion and MNase ChIP-seq to investigate nucleosome dynamics and interactions with chromatin-related factors.
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