[DETERMINATION OF PHYLOGENETIC RELATIONSHIP OF YERSINIA PESTIS STRAINS FROM NATURAL PLAGUE FOCI OF THE CAUCASUS BY MULTI-LO- CUS VNTR-ANALYSIS].

Aim: Determination of the degree of phylogenetic relationship of Yersinia pestis strains iso- lated from the territories of natural foci of plague from the Caucasus using VNTR-typing by 25 loci (MLVA25).

Materials And Methods: 26 strains of Y pestis from Russian natural foci of the Caucasus were used in the study. 25 loci of tandem repeats in Y pestis genome by Le Fleche scheme were used for execution of multi-locus VNTR-analysis.

[The duration of preservation of plague pathogen strains with a different plasmid kit from a Central Caucasian high-altitude natural focus in the organism of citellophilus tesquorum fleas and the possibility of their transmission to laboratory animals].

Two plasmid variants of the main subspecies of the plague microbe circulate in a Central Caucasian high-altitude natural focus of plague. The strains of one plasmid variant fully correspond to the main subspecies of the plague pathogen in their characteristics. Those of the other are auxotrophic for proline, weakly virulent to one or both species of laboratory animals.

[New preparations for the rapid diagnostics of dangerous infectious diseases and their use in emergency situations].

The use of new diagnostic preparations (magnetic immunosorbents, polyvalent fluorescent immunoglobulins and immunoperoxidase conjugates), as well as the combination of magnetic immunosorbents with the polymerase chain reaction, for the rapid diagnostics of dangerous infectious diseases has been shown to have good prospects. Information on their successful use for laboratory diagnostics in emergency situations is presented.

[Comparative characterization of Vibrio cholerae O1, isolated from surface water reservoirs in Grozny in 1995 and 2000].

The results of complete identification revealed the phenotypical similarity of V. cholerae strains isolated from surface water reservoirs im Grozny in 1995 and 2000. The strains under study proved to be atoxigenic, hemolysis-positive and phage resistant.

[Laboratory diagnostics of acute enteric infections under the conditions of the work of the specialized epidemic brigade during liquidation of the consequences of the emergency situations].

The scheme of the complex microbiological diagnostics of cholera, dysentery, salmonellosis and escherichiosis has been developed. The scheme includes the introduction of material taken from a patient with acute enteric infection into 1% peptone water (pH 8.0), followed by inoculation into Endo medium (pH 7.