Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium T1Kr02, Containing d-Fucose Residues.

Lipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical-chemical properties and biological activity of LPS were also investigated. The NMR analysis showed that the O-polysaccharide has the following structure: →2)-β-d-Fuc-(1→3)-β-d-Fuc-(1→.

Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus.

Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain.

Phenotyping of lymphoproliferative tumours generated in xenografts of non-small cell lung cancer.

Background: Patient-derived xenograft (PDX) models involve the engraftment of tumour tissue in immunocompromised mice and represent an important pre-clinical oncology research method. A limitation of non-small cell lung cancer (NSCLC) PDX model derivation in NOD- IL2Rgamma (NSG) mice is that a subset of initial engraftments are of lymphocytic, rather than tumour origin.

Methods: The immunophenotype of lymphoproliferations arising in the lung TRACERx PDX pipeline were characterised.

[DNA Fragment Enrichment for High-Throughput Sequencing].

This review describes the application of oligonucleotides, which are mainly obtained using DNA synthesizers of a new generation (microarray DNA synthesizers), for the enrichment of target genomic fragments. The methods of molecular hybridization, polymerase chain reaction, and CRISPR-Cas9 system for this purpose are considered. Examples of the practical use of the developed methods for research and diagnostic purposes are given.

Paleophytogeography of the Siberian Paleofloristic Region in the Early Jurassic and First Half of the Middle Jurassic.

The results of a comparative analysis of taphofloras of the Early Jurassic and first half of the Middle Jurassic of the Siberian paleofloristic region are considered. For this time interval, a significant similarity in the systematic composition of the taphofloras of Western Siberia and Northern China is revealed. Based on the author's data and the published data, the position of the boundary in the south and southwest of the Siberian paleofloristic region and the boundary between its West Siberian and North China provinces are corrected.