Publications by authors named "E B Buckner"

Genetic screens in have long been used to identify genes found in a variety of developmental, cellular, and behavioral processes. Here we describe the characterization and mapping of a mutation identified in a conditional screen for genetic regulators of cell growth and cell division. Within a Flp/FRT system, mutant results in a reduction of mutant tissue and a rough eye phenotype.

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Article Synopsis
  • * The In2Care Mosquito Station, which uses pyriproxyfen (PPF) and Beauveria bassiana spores, has shown effectiveness in controlling other mosquitoes and was tested for its potential against Cx. quinquefasciatus.
  • * Results from semifield studies indicate that the In2Care station attracts female Cx. quinquefasciatus, leading to successful PPF autodissemination and reduced mosquito survival, suggesting it could help control this mosquito species, though further field studies are necessary.
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Aedes aegypti is an important vector of dengue virus and other arboviruses that affect human health. After being ingested in an infectious bloodmeal, but before being transmitted from mosquito to human, dengue virus must disseminate from the vector midgut into the hemocoel and then the salivary glands. This process, the extrinsic incubation period, typically takes 6-14 days.

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In the last 2 decades, there has been an increase in the geographic range and frequency of vector-borne diseases. Management of mosquito populations has become challenging due to increasing rates of resistance to existing insecticidal products and formulations. Several alternative tools have emerged to suppress or replace mosquito populations.

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The CDC Biofilm Reactor method is the standard biofilm growth protocol for the validation of US Environmental Protection Agency biofilm label claims. However, no studies have determined the effect of coupon orientation within the reactor on biofilm growth. If positional effects have a statistically significant impact on biofilm density, they should be accounted for in the experimental design.

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