[The HPLC analysis of vanillil-almond, 5-hydroxiindolacetic, homovanillic and homogentisic acids in biologic fluids in clinical laboratory].

The simple and fast HPLC technique of analysis of vanillil-almond, 5-hydroxiindolacetic, homovanillic and homogentisic acids in urine with solid-phase extraction using ultra cross-linked polystyrene (Purosep-200) is proposed. The separation on column Chromolith Performance RP-18e "Merck" 100x4.6 mm with monolithic phase-reversed silica gel in isocratic or gradient mode with ultraviolet detection under 285 nm.

[HPLC for the diagnosis and monitoring of phenylketonuria and ketoaciduria].

A simple, rapid, and sensitive HPLC is proposed to test six amino acids in plasma/serum. Deproteinization was carried out with acetonitrile; derivation was made with the orthophthalic aldehyde 2-mercaptoethanol. Separation was accompanied on a Chromolith (Merck), 4.

[Infusion therapy in sessions of general artificial hyperthermia using large doses of insulin in cancer patients].

A scheme of infusion correction of water-electrolyte shifts occurring during general artificial hyperthermia sessions (rectal t degrees-40-42 degrees C) employing large insulin doses (100-1120 U) in cancer patients has been suggested. The infusion therapy according to the technique described was used during 103 general artificial hyperthermia sessions in 58 patients. This made it possible to maintain physiologically normal blood plasma levels of glucose, Na+, K+, calcium, inorganic phosphates, normal acid-base balance, adequate hydration and circulating blood volume.