The influence of folate supplementation on global gene expression in normal colonic mucosa of subjects with colorectal adenoma.

Scope: We determined whether short-term supplementation with a physiological dose of folate alters global gene expression in the normal colonic mucosa of subjects with colorectal adenoma.

Methods And Results: Fourteen subjects with adenoma, randomised to receive folic acid (400μg/day, n = 6) or placebo (n = 8) for 10 weeks, had blood samples and colonic tissue biopsies collected before and after the intervention. RNA extracted from colonic tissue samples was used to determine global gene expression in the colon using Affymetrix® Microarray GeneChips and real-time RT-PCR.

Effect of high-fat feeding on expression of genes controlling availability of dopamine in mouse hypothalamus.

Objective: Hypothalamic centers integrate external signals of nutrient availability and energy status and initiate responses to maintain homeostasis. Quantifying changes in hypothalamic gene expression in the presence of nutrient excess may identify novel responsive elements.

Methods: Affymetrix Mouse Genome 430 2.