Growth and adherence on stainless steel by Enterococcus faecium cells.

Enterococcus faecium isolated from Brazilian raw milk was used in this study. For growth studies, E. faecium was inoculated into 10% RSM (reconstituted skim milk) and MRS both, incubated at 6.

Bacteriocidal activity of sanitizers against Enterococcus faecium attached to stainless steel as determined by plate count and impedance methods.

Enterococcus faecium attached to stainless steel chips (100 mm2) was treated with the following sanitizers: sodium hypochlorite, peracetic acid (PA), peracetic acid plus an organic acid (PAS), quaternary ammonium, organic acid, and anionic acid. The effectiveness of sanitizer solutions on planktonic cells (not attached) was evaluated by the Association of Official Analytical Chemists (AOAC) suspension test. The number of attached cells was determined by impedance measurement and plate count method after vortexing.

Inhibition of Listeria monocytogenes , Escherichia coli O157:H7, and Micrococcus luteus by Linear Furanocoumarins in Culture Media.

The linear furanocoumarins psoralen, 5-methoxypsoralen (5-MOP), and 8-methoxypsoralen (8-MOP) were tested as antimicrobial agents against Listeria monocytogenes , Escherichia coli O157:H7 and Micrococcus luteus . The linear furanocoumarins were activated with UV light at 365 nm for 60 min. MICs of these furanocoumarins in tryptic soy broth supplemented with 0.

Inhibition of Listeria monocytogenes , Escherichia coli O157:H7, and Micrococcus luteus by Linear Furanocoumarins in a Model Food System.

Lime peel, parsnip, lemon peel, dried parsley flakes, cold pressed lime oil, and distilled lime oil samples were analyzed for the presence and concentration of the linear furanocoumarins (LFs) psoralen, 5-methoxypsoralen (5- MOP), and 8-methoxypsoralen (8-MOP) by thin layer chromatography and gas chromatography mass spectrometry. Cold-pressed lime oil had the highest LF content (psoralen, 67 ± 29 μg/ml, 5-MOP, 1,634 ± 62 μg/ml, and 8-MOP, 44 ± 2 μg/ml). The antimicrobial effectiveness of LFs against Listeria monocytogenes , Escherichia coli O157:H7, and Micrococcus luteus was tested in a model food system consisting of a slurry of 25% commercial "garden vegetables" baby food in 0.

Isolation and Identification of Adherent Gram-Negative Microorganisms from Four Meat-Processing Facilities.

Biofilms are described as a matrix of microorganisms which have adhered to and colonized a surface. Once formed, biofilms are difficult to remove and may be a source of contamination in food-processing environments. In this study, stainless-steel chips were fixed to surfaces adjacent to food-contact surfaces and cast-iron chips were suspended in the floor drains of four meat-processing plants.