Publications by authors named "E A Vuono"
Article Synopsis
- The classical swine fever virus (CSFV) has three important glycoproteins, with E2 playing a crucial role in virus attachment to host cells and overall virulence.
- Research confirmed that E2 interacts with the scaffolding protein DOCK7 in swine cells using techniques like co-immunoprecipitation and proximity ligation assays, identifying key amino acid residues essential for this interaction.
- Mutations that disrupted the E2-DOCK7 interaction were introduced into a recombinant CSFV strain, but the modified virus still exhibited similar growth and virulence characteristics as the original strain, suggesting that E2's interaction with DOCK7 is not essential for virus replication or disease progression.
African swine fever (ASF) is a devastating disease that is currently producing a panzootic significantly impacting the swine industry worldwide. One of the major challenges for advancing the development of ASF vaccines has been the absence of international standards for ASF vaccine purity, potency, safety, and efficacy. To date, the most effective experimental vaccines have been live attenuated strains of viruses.
View Article and Find Full Text PDFThe E2 glycoprotein is one of the four structural proteins of the classical swine fever virus (CSFV) particle. E2 has been shown to be involved in many virus functions, including adsorption to host cells, virus virulence and interaction with several host proteins. Using a yeast two-hybrid screen, we have previously shown that the CSFV E2 specifically interacts with swine host protein medium-chain-specific acyl-Coenzyme A dehydrogenase (ACADM), an enzyme that catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway.
View Article and Find Full Text PDFAfrican swine fever virus (ASFV) is the etiological agent of an economically important disease of swine currently affecting large areas of Africa, Eurasia and the Caribbean. ASFV has a complex structure harboring a large dsDNA genome which encodes for more than 160 proteins. One of the proteins, E66L, has recently been involved in arresting gene transcription in the infected host cell.
View Article and Find Full Text PDFAfrican swine fever is a lethal disease of domestic pigs, geographically expanding as a pandemic, that is affecting countries across Eurasia and severely damaging their swine production industry. After more than 40 years of being absent in the Western hemisphere, in 2020 ASF reappeared in the Dominican Republic and Haiti. The recent outbreak strain in the Dominican Republic has been identified as a genotype II ASFV a derivative of the ASF strain circulating in Asia and Europe.
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