Cell and molecular determinants of in vivo efficacy of the BH3 mimetic ABT-263 against pediatric acute lymphoblastic leukemia xenografts.

Purpose: Predictive biomarkers are required to identify patients who may benefit from the use of BH3 mimetics such as ABT-263. This study investigated the efficacy of ABT-263 against a panel of patient-derived pediatric acute lymphoblastic leukemia (ALL) xenografts and utilized cell and molecular approaches to identify biomarkers that predict in vivo ABT-263 sensitivity.

Experimental Design: The in vivo efficacy of ABT-263 was tested against a panel of 31 patient-derived ALL xenografts composed of MLL-, BCP-, and T-ALL subtypes.

MicroRNA control of myelopoiesis and the differentiation block in acute myeloid leukaemia.

In the relatively short period of time since their discovery, microRNAs have been shown to control many important cellular functions such as cell differentiation, growth, proliferation and apoptosis. In addition, microRNAs have been demonstrated as key drivers of many malignancies and can function as either tumour suppressors or oncogenes. The haematopoietic system is not outside the realm of microRNA control with microRNAs controlling aspects of stem cell and progenitor self-renewal and differentiation, with many, if not all, haematological disorders associated with aberrant microRNA expression and function.

Circadian rhythms of gingival epithelium in young mice.

Five-week-old Brookhaven National Laboratory (BNL), Swiss-albino male mice were maintained on a 12-hour light/dark cycle and were subcutaneously injected with 3H-thymidine (1 micro Ci/gm body weight) one hour prior to sacrifice. Twenty-four mice (three each time-period) were killed at 3 hour intervals for 24 hours. Autoradiographs were prepared from 5-micron thick paraffin embedded decalcified sections of maxillary first molars and surrounding tissues.

Autoradiographic investigation of circadian rhythms in alveolar bone periosteum and cementum in young mice.

This report presents circadian rhythms in cell proliferation of alveolar bone periosteum and cementum of the maxillary first molars of male 5-week-old BNL, Swiss albino mice which were maintained on a 12 hr light/dark cycle. Mice were injected with 3H-TDR (luCi/gm. body wt.