[Activation of adenyl cyclase from bovine brain caudate nucleus by synthetic analogs of guanyl nucleotides].

In order to investigate the regulatory properties of dopamine-sensitive adenylate cyclase from bovine brain caudate nucleus some non-hydrolyzed guanyl nucleotides modified at the 8th position of the purine base and at phosphate site were synthesized. The activating effects of these compounds and that of GppCH2p on the membrane preparation of adenylate cyclase were studied. The activating effect of GppCH2p was decreased when the substituents were introduced at the 8th position of the purine base or when its terminal phosphate group was modified.

[Irreversible inhibition of adenylate cyclase by oxo-ATP].

Adenylate cyclase from rabbit heart membranes is irreversibly inhibited by 2',3'-dialdehyde of ATP (oxo-ATP). The inhibiting effects is observed during membrane incubation with the inhibitor. Sodium borohydride increase the degree of the enzyme inactivation by oxo-ATP.

[Adenylate cyclase from rabbit heart: substrate binding site].

The effects of 17 ATP analogs on the solubilized rabbit heart adenylate cyclase were studied. The triphosphate chain, position 8 of the adenine base and the ribose residue of the ATP molecule were modified. Despite the presence of the alkylating groups in two former types of the analogs tested, no covalent blocking of the active site of the enzyme was observed.

[Ways of determination of the intensity of the epidemic process in Shigella sonnei dysentery].

Proper view on the true prevalence of Sonne dysentery characterised by polymorphous clinical picture in which many cases coursed in subclinical form could be reached only by using additional active methods for detecting the infection rate of the population. For this purpose the authors applied passive hemagglutination test which permitted to reveal the response of the organism to the antigenic stimulation in the course of two months after the sustained sickness. Over 12 000 persons were examined.