[IMMUNOCHEMICAL ANALYSIS OF RECOMBINANT CHIMERIC POLYPEPTIDE OspC(gar+afz) OF BORRELIA GARINIIANIP B. AFZELI ISOLATES].

Aim: Comparative study of antigenic properties of recombinant proteins OsPCgar and OsPCafz and recombinant chimeric polypeptide OspCgar+afrz, that contains amino acid sequences of mature immune dominant OspC proteins of West-Siberian isolates of Borrelia garinii (OspCgar) and B. afzelii (OspCafz), and evaluation of possibility of their use as antigens during creation of test-systems for serodiagnostics of Lyme borreliosis (LB) on the territory of Western Siberia.

Materials And Methods: Recombinant chimeric polypeptide OSpCgar+af, and recombinant mature proteins OSPCgar and OspCafz, obtained by expression of the corresponding genes in Escherichia coli cells; purified by affinity chromatography in Ni-NTA-sepharose CL-6B and studied by EIA method for the ability to bind antibodies from sera of LB patients.

[Surgical prophylaxis of pulmonary embolism].

144 patients were operated on for deep vein thrombosis. Color duplex lower limb vessels scanning was he basic diagnostic procedure performed preoperatively. Operative treatment corresponded floating trombectomy and main vein placation as a means of pulmonary embolism prophylaxis.

[Invasive treatment of acute coronary syndrome in multiple affection of the coronary arteries].

A comparative assessment of the risk of invasive myocardial revascularizations in patients with multiple affection of coronary arteries and acute coronary syndrome (ACS) without stable ST-elevation on ECG was carried out. Group A (n=45) consisted of patients with one-stage revascularization with coronary bypass (CB) during ACS, group B (n=45) - balloon angioplasty /stenting of symptom-determining artery with subsequent CB in the "cold period" (6 months after ACS episode). It is demonstrated that the one-stage procedure (i.