Immunolocalization of cell-wall-deficient forms of Mycobacterium tuberculosis complex in sarcoidosis and in sinus histiocytosis of lymph nodes draining carcinoma.

In sarcoidosis, pleomorphic chromogens (PCs) occur as multivariate pigmented elements within sinusoids of lymph nodes (sinusoidal phase) and as tiny "round bodies" detectable in granulomas (generalized phase). The sinusoidal phase occurs in other conditions as well and characteristically contains yeastlike bodies also known as H-W bodies. To elucidate the antigenic profile of all variant forms, 28 cases of sarcoidosis (series A) and 14 cases of malignancy associated sinus histiocytosis (series B) were studied immunohistochemically with panels of various antibodies, including antimycobacterial MAbs specific for M tuberculosis complex (TB68, TB71), for M.

Cross-reactive and species specific Mycobacterium tuberculosis antigens in the immunoprofile of Schaumann bodies: a major clue to the etiology of sarcoidosis.

Sarcoidosis, once thought to be a variant of tuberculosis, is currently listed as a disease of unknown etiology. The present study was initiated by unpublished observations that Schaumann bodies-the laminated inclusions often encountered in sarcoid granulomas-cross-reacted with commercial polyclonal antibodies to Mycobacterium bovis, Mycobacterium duvalii and Mycobacterium paratuberculosis. Given the broad cross-reactivity of many mycobacterial antigens, those findings lacked specificity but warranted in depth probing of the immunoprofile of the bodies, particularly for specific mycobacterial antigens.

Asteroid bodies: products of unusual microtubule dynamics in monocyte-derived giant cells. An immunohistochemical study.

We have studied asteroid bodies (ABs) of multinucleated giant cells (MGCs) in a series of sarcoid and foreign body granulomas with a standard streptavidin-biotin peroxidase technique, using commercial antibodies against collagen, vimentin and tubulin on routinely processed tissue as well as, in one case, on fresh frozen sections (FS). Our findings clearly indicate that ABs are products of the microtubule (MT) system and lack collagen. The tubulin in them stains in fresh FS but is "masked" in formalin-fixed tissue.

Selective immunolabeling of early gestational cytotrophoblast and its neoplastic counterpart by the monoclonal antibody Ber-EP4.

This report presents preliminary observations on the distribution of an epithelial surface antigen in human trophoblastic cells studied with the monoclonal antibody Ber-EP4 by means of an immunoperoxidase technique. Three cases of gestational choriocarcinoma and 20 cases of both villous and extravillous trophoblast, including one complete hydatidiform mole and one exaggerated placental site, were examined with a panel of monoclonal antibodies against epithelial antigen (Ber-EP4), cytokeratin (AE1 and AE3), vimentin (VIM), carcinoembryonic antigen (CEA) and epithelial membrane antigen (EMA), as well as with polyclonal antibodies against the beta subunit of human chorionic gonadotropin (hCG), human placental lactogen (hPL) and placental alkaline phosphatase (P1AP). While, as anticipated, all currently defined trophoblastic cell lines expressed low molecular weight cytokeratin only first trimester cytotrophoblastic cells and their neoplastic counterpart were found to possess the epithelial antigen detected by Ber-EP4.