Publications by authors named "E A Kolosovskaya"
In barley having adherent hulls, an irreversible connection between the pericarp with both palea and lemma is formed during grain maturation. A mutation in the () gene prevents this connection and leads to the formation of barley with non-adherent hulls. A genetic model of two isogenic lines was used to elucidate the genetic mechanisms of hull adhesion: a doubled haploid line having adherent hulls and its derivative with non-adherent hulls obtained by targeted mutagenesis of the gene.
View Article and Find Full Text PDFgenes () and () play a regulatory role in cuticle organization. Because the cuticle is a key evolutionary acquisition of plants for protection against environmental factors, a knockout (KO) of each gene may alter their ability to adapt to unfavorable conditions. A potential pleiotropic effect of or gene mutations can be assessed under salt stress.
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- Plant surface properties are vital for coping with environmental stresses, and the cuticle's outer layer is made up of diverse waxes influenced by specific gene regulators.
- In this study, researchers used a technique called RNA-guided Cas9 endonuclease to create mutations in four barley genes linked to cuticle properties, identifying one gene responsible for a glossy sheath phenotype due to a deficiency in β-diketones.
- The results indicated that the WIN1 transcription factor plays a crucial role in regulating the biosynthesis of β-diketones in barley by activating certain genes during specific developmental stages and in particular plant organs.
The genes and play a regulatory role in cuticle organization in barley. A knockout (KO) of each gene may alter plant mechanisms of adaptation to adverse environmental conditions. A putative pleiotropic effect of or gene mutations in barley can be assessed in a series of experiments in the presence or absence of a provoking factor.
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- The naked caryopsis trait in barley results from a loss-of-function mutation in the NUD gene, which is crucial for forming a cementing layer in the grain.
- Researchers used RNA-guided Cas9 to effectively knock out the NUD gene, leading to a 57% conversion rate from hulled to naked barley in transformed plants.
- The development of isogenic lines of hulled and naked barley offers an ideal model for studying the effects of NUD mutations on plant performance and yield, with naked barley having health benefits due to its high β-glucan content.