[Activity of pollulan synthesis by diploid culture of Pollularia pollulans on media with different sources of carbon].

The diploid culture of Pullularia pullulans 1125(4)(13) synthesizes pullulan from different carbon sources. The activity is highest on a mineral medium with melassa (23.18 g/L).

[Carbohydrates of the medium and the nature of ontogeny in Aspergillus awamori in relation to glucoamylase biosynthesis].

The biological rhythm of the transition from the processes of blocking and nuclear division to active RNA biosynthesis and growth in submerged culture of Aspergillus awamori producing glucoamylase was studied by the technique of quantitative cytochemistry, and was characterized by distinct daily oscillation in a medium with starch. The rhythm was disturbed when other carbon sources, viz. maltose, sucrose and particularly glucose, were used: the rhythm became longer and uneven, the rate of nuclear blocking decreased, and the de novo formation of the mycelium of the adaptive stage capable of producing glucoamylase was inhibited.

[Study of the conditions maintaining the synthesis of alpha-amylase by Bacillus species].

The effect of temperature and different carbon sources on the growth of Bacillus species and the synthesis of alpha-amylase was studied. The temperature of 40 degrees proved to be optimal for those processes. Starch was found to be the best carbon source.