Functional modification of a murine macrophage cell line, J744A.1, transfected with rat csk genes.

In a previous study of this laboratory, a macrophage cell line, J774A.1, transfected with rat csk gene and overexpressing the Csk proteins has been established. These Csk transfectants showed depressed productions of monokines and nitric oxide (NO), but enhanced production of prostaglandin E2 (PGE2).

Delayed clearance of zymosan-induced granuloma and depressed phagocytosis of macrophages with concomitant up-regulated kinase activities of Src-family in a human monocyte chemoattractant protein-1 transgenic mouse.

A human monocyte chemoattractant protein-1 (hMCP-1) transgenic mouse (Tgm) line which constitutively produces a large amount of hMCP-1 (7-13 ng/ml in the serum) was established. Although expression of the transgene was detected in various tissues, an accumulation of macrophages (Mphi) was seen in only lymphoid organs which might be attributed to the high concentration of hMCP-1 in these organs. A reduced phagocytosis by peritoneal Mphi in vivo and a delayed clearance of granulomas in the liver following zymosan administration were observed in these Tgm.