In comparison to progenitor platelets, microparticles are deficient in GpIb, GpIb-derived carbohydrates, glycerophospholipids, glycosphingolipids, and ceramides.

Activated blood platelets shed microparticles with procoagulant activity that probably participate in normal hemostasis. We have isolated spontaneously formed microparticles from human blood and analysed them for ultrastructure, antigenic profile, and biochemical composition. In transmission electron microscopy microparticles appeared as regular vesicles with a mean diameter of 300 nm (50-600 nm).

[Comparison of platelet and leukocyte content in platelet concentrates obtained from CS-3000 and third generation (CS-3000 plus and Cobe-Spectra) cell separators].

Platelet concentrates (PCs) obtained using old generation of cell separators contain high number of leukocytes. White cell (WBC) contamination and platelet (Plts) number in PCs obtained from separator II-nd generation CS-3000 and separators III-rd generations CS-3000 plus and Cobe-Spectra have been determined. PCs from new separators contain the same Plts number as PCs from CS-3000.

[Evaluation of platelet quality obtained using conventional methods or from buffy-coat and stored at room temperature].

A comparison has been made between platelet concentrates (Pcs) obtained from buffy coat (Bc) and from platelet rich plasma. The method of preparation proves to have an platelet properties in vitro. Both types of Pcs were investigated immediately after isolation as well as during the 5 day period of storage.

[Comparison of platelet concentrates obtained using blood cell separators with continuous flow: CS-3000 and Cobe-Spectra].

During a platelet apheresis procedure on CS-3000 the platelet concentrate (Pc) is centrifugated for about 1.5 h. In the Cobe system Pc is gradually collected in a container located outside the machine.

[Platelet transfusion from HPA typed donors in alloimmune thrombocytopenia of newborns and fetuses].

The registry of donors typed for HPA-1 and HPA-3 antigens is presented. Three cases of fetal/neonatal alloimmune thrombocytopenia (F/NAIT) transfused with typed platelets either from mother or from registered donors are discussed. All children were transfused just after delivery, one in addition in utero: they survived not having any haemorrhagic complications in central nervous system inspite of such complications in previous born siblings.

[Sialic acid level in blood platelets after cryopreservation with DMSO].

Plasma platelet concentrates of man were subjected to preparatory procedures with dimethylsulphoxide (DMSO) as a cryoprotective agent. It was demonstrated that DMSO acting directly on the platelets in concentrations used in routine preparation had no effect decreasing the level of sialic acids in the platelets. Cryopreservation of platelets in presence of DMSO caused the loss of 30% of the sialic acids from the platelets.