Serodiagnostics of Toxoplasma gondii invasions.

Diagnosis of T. gondii invasions is based mainly on serological tests, because of their relative simplicity and availability. In the present work we examined efficiency of immunoblot and two inhouse ELISA assays in anti-Toxoplasma antibody detection, comparing our results with those obtained using commercial ELISA kit of Organon Teknika.

[Toxoplasma gondii proteome].

The article presents data concerning methods of proteomics and main achievements of studies on Toxoplasma gondii proteom.

[Antigens of Toxoplasma gondii].

The paper presents antigenic structure of T. gondii tachyzoites and bradyzoites in respect of potential use of some chosen antigens for diagnostic and immunoprophylactic purposes.

[Identification of Toxoplasma gondii antigens recognized by specific T lymphocytes].

The paper presents chosen experimental strategies for identifying of Toxoplasma gondii antigens recognized by specific T lymphocytes.

[Antigens of Toxoplasma gondii].

The paper presents antigenic structure of T. gondii tachyzoites and bradyzoites in respect of potential use of some chosen antigens for diagnostic and immunoprophylactic purposes.

Specific anti-Toxoplasma gondii antibodies produced in inbred mice differring in their natural resistance to toxoplasmosis.

The laboratory diagnostics of T. gondii infections both in humans and animals relies mainly upon detection of specific antibodies. We studied the influence of the host genetic factors on the level and repertoire of antibodies produced in Toxoplasma infection employing as an experimental model two inbred mouse strains with different innate susceptibility to toxoplasmosis.

Epidermal growth factor enhances TNF-alpha-induced priming of human neutrophils.

The intensity of neutrophil inflammatory response could be rapidly amplified by priming with pro-inflammatory mediators such as TNF-alpha, GM-CSF or LPS at low concentrations prior to stimuli. We proposed that epidermal growth factor (EGF) increases TNF-alpha-induced priming of human neutrophils. This study showed that EGF enhanced TNF-alpha-induced activation of neutrophils functions.

Towards the Toxoplasma gondii proteome: position of 13 parasite excretory antigens on a standardized map of two-dimensionally separated tachyzoite proteins.

High resolution two-dimensional separation of Toxoplasma gondii tachyzoite lysate revealed up to 224 distinct protein spots in Coomassie-stained gel. Computional matching of 14 digitized gels yielded a standard two-dimensional proteome map. The excretory T.

Attenuation of mouse-virulent Toxoplasma gondii parasites is associated with a decrease in interleukin-12-inducing tachyzoite activity and reduced expression of actin, catalase and excretory proteins.

Determinants of Toxoplasma gondii virulence are still unknown, although genetic markers associated with T. gondii pathogenicity or host susceptibility to infection have been identified. To define indicator proteins of mouse virulence, type I strain parasites were attenuated by continuous passage in fibroblast culture and compared with the parental strain passaged in mice.