Publications by authors named "Dylan Matvey"
Purpose: Oncogene-driven macropinocytosis fuels nutrient scavenging in some cancer types, yet whether this occurs in thyroid cancers with prominent MAPK-ERK and PI3K pathway mutations remains unclear. We hypothesized that understanding links between thyroid cancer signaling and macropinocytosis might uncover new therapeutic strategies.
Experimental Design: Macropinocytosis was assessed across cells derived from papillary thyroid cancer (PTC), follicular thyroid cancer (FTC), non-malignant follicular thyroid, and aggressive anaplastic thyroid cancer (ATC), by imaging fluorescent dextran and serum albumin.
BRAF-targeted kinase inhibitors (KIs) are used to treat malignancies including BRAF-mutant non-small cell lung cancer, colorectal cancer, anaplastic thyroid cancer, and, most prominently, melanoma. However, KI selection criteria in patients remain unclear, as are pharmacokinetic/pharmacodynamic (PK/PD) mechanisms that may limit context-dependent efficacy and differentiate related drugs. To address this issue, we imaged mouse models of BRAF-mutant cancers, fluorescent KI tracers, and unlabeled drug to calibrate in silico spatial PK/PD models.
View Article and Find Full Text PDFConfocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer.
Methods Mol Biol
January 2022
Article Synopsis
- Ovarian cancer is often diagnosed late, with tumors spreading throughout the abdominal cavity and ascitic fluid.
- Confocal microscopy techniques are introduced to study ovarian cancer in its natural tissue environment using mouse models, allowing detailed imaging at the single-cell level.
- The study demonstrates simultaneous imaging of tumor blood vessels, immunosuppressive immune cells, and ovarian cancer kinase activity to better understand the disease progression.