Sialylation regulates neutrophil transepithelial migration, CD11b/CD18 activation, and intestinal mucosal inflammatory function.

Polymorphonuclear neutrophils (PMNs) play a critical role in clearing invading microbes and promoting tissue repair following infection/injury. However, dysregulated PMN trafficking and associated tissue damage is pathognomonic of numerous inflammatory mucosal diseases. The final step in PMN influx into mucosal lined organs (including the lungs, kidneys, skin, and gut) involves transepithelial migration (TEpM).

High-Yielding Automated Convergent Synthesis of No-Carrier-Added [C-]-Labeled Amino Acids Using the Strecker Reaction.

A new variant of the Strecker synthesis using no-carrier-added [C]cyanide for the synthesis of radiolabeled amino acids is described. The protocol is fully automated using a radiochemistry synthesis module and applied to the production of a number of new PET radiotracers. [C-]sarcosine, [C-]methionine, [C-]--phenylglycine, and [C-]glycine are all synthesized in moderate to good radiochemical yields.