Determination of Cassiarin A Level of Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method.

leaf has been proven and to have a strong antimalarial activity with Cassiarin A as its active compound. To obtain a source of medicinal plant with high level of active antimalarial compound (Cassiarin A), a valid method for determining Cassiarin A level is needed. For this reason, this research conducts the validation of the Cassiarin A content with determination method using thin-layer chromatography (TLC) densitometry which includes the determination of selectivity (s), linearity (r), accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ).