Identification and Analysis of , , , and Gene Families in .

(β-ketoacyl-acyl carrier protein (ACP) synthases II), (fatty acid thioesterases), (stearoyl-ACP desaturase), and (fatty acid desaturases) are the vital gene families involved in fatty acid (FA) synthesis in L. However, information on the number and location of these genes and which ones are key to the formation of FAs in fruit seeds and pulp was not complete. Our study aimed to solve this issue using the available genomic sequences and transcriptome data that we obtained.

Nanopore Data-Driven Chromosome-Level Assembly of Flax Genome.

Flax is an important crop grown for seed and fiber. Flax chromosome number is 2n = 30, and its genome size is about 450-480 Mb. To date, the genomes of several flax varieties have been sequenced and assembled.

Nanopore Data-Driven T2T Genome Assemblies of Strains.

is a pathogenic fungus that infects flax and causes significant yield losses. In this study, we assembled the genomes of four highly virulent strains using the Oxford Nanopore Technologies (ONT, R10.4.

Expression of and Genes in Developing Seeds of Flax Varieties under Different Growth Conditions.

Flax seed is one of the richest plant sources of linolenic acid (LIN) and also contains unsaturated linoleic acid (LIO) and oleic acid (OLE). Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play key roles in the synthesis of flax fatty acids (FAs). However, there is no holistic view of which genes from the and families and at which developmental stages have the highest expression levels in flax seeds, as well as the influence of genotype and growth conditions on the expression profiles of these genes.

Comparative Genomic Analysis of Strains with Different Virulence on Flax.

is a flax fungal pathogen. The genus comprises differently virulent strains, leading to significant yield losses. However, there were no attempts to investigate the molecular mechanisms of pathogenicity from high-quality genome assemblies until this study.

ITS and 16S rDNA metagenomic dataset of different soils from flax fields.

Flax ( L.), one of the important and versatile crops, is used for the production of oil and fiber. To obtain high and stable yields of flax products, varieties should be cultivated under optimal conditions, including the composition of the soil microbiome.

Selection of Flax Genotypes for Pan-Genomic Studies by Sequencing Tagmentation-Based Transcriptome Libraries.

Flax ( L.) products are used in the food, pharmaceutical, textile, polymer, medical, and other industries. The creation of a pan-genome will be an important advance in flax research and breeding.

Sex-determining region complements traditionally used in phylogenetic studies nuclear and chloroplast sequences in investigation of Duby and Spach poplars (genus L., Salicaceae).

Members of the genus L. play an important role in the formation of forests in the northern hemisphere and are used in urban landscaping and timber production. species of closely related sections show extensive hybridization.

Key , , and Genes Involved in the Fatty Acid Synthesis in Flax Identified Based on Genomic and Transcriptomic Data.

(fatty acid desaturase) and (stearoyl-ACP desaturase) genes play key roles in the synthesis of fatty acids (FA) and determination of oil composition in flax ( L.). We searched for and genes in the most widely used flax genome of the variety CDC Bethune and three available long-read assembled flax genomes-YY5, 3896, and Atlant.

A quantitative characterization of early neuron generation in the developing zebrafish telencephalon.

The adult brain is made up of anatomically and functionally distinct regions with specific neuronal compositions. At the root of this neuronal diversity are neural stem and progenitor cells (NPCs) that produce many neurons throughout embryonic development. During development, NPCs switch from initial expanding divisions to neurogenic divisions, which marks the onset of neurogenesis.

Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization.

The naked mole-rat (NMR) is a unique long-lived rodent which is highly resistant to age-associated disorders and cancer. The immune system of NMR possesses a distinct cellular composition with the prevalence of myeloid cells. Thus, the detailed phenotypical and functional assessment of NMR myeloid cell compartment may uncover novel mechanisms of immunoregulation and healthy aging.

Assembling Quality Genomes of Flax Fungal Pathogens from Oxford Nanopore Technologies Data.

Flax ( L.) is attacked by numerous devastating fungal pathogens, including , , and (). The effective control of flax diseases follows the paradigm of extensive molecular research on pathogenicity.

Development and Complex Application of Methods for the Identification of Mutations in the and Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil.

Flax is grown worldwide for seed and fiber production. Linseed varieties differ in their oil composition and are used in pharmaceutical, food, feed, and industrial production. The field of application primarily depends on the content of linolenic (LIN) and linoleic (LIO) fatty acids.

Isolating L. Nuclear DNA Enabled Assembling High-Quality Genome.

High-quality genome sequences help to elucidate the genetic basis of numerous biological processes and track species evolution. For flax ( L.)-a multifunctional crop, high-quality assemblies from Oxford Nanopore Technologies (ONT) data were unavailable, largely due to the difficulty of isolating pure high-molecular-weight DNA.

Clarification of the Position of Planch. within the Phylogeny of the Genus L.

The phylogeny of members of the family DC. ex Perleb has not been adequately studied. In particular, data on the phylogenetic relationship between Planch.

Genes Associated with the Flax Plant Type (Oil or Fiber) Identified Based on Genome and Transcriptome Sequencing Data.

As a result of the breeding process, there are two main types of flax ( L.) plants. Linseed is used for obtaining seeds, while fiber flax is used for fiber production.

Genome Assembly and Sex-Determining Region of Male and Female × .

The genus is presented by dioecious species, and it became a promising object to study the genetics of sex in plants. In this work, genomes of male and female × individuals were sequenced for the first time. To achieve high-quality genome assemblies, we used Oxford Nanopore Technologies and Illumina platforms.

Genome and Transcriptome Sequencing of × Identified Sex-Associated Allele-Specific Expression of the Gene.

Transcriptome sequencing of leaves, catkin axes, and flowers from male and female trees of × and genome sequencing of the same plants were performed for the first time. The availability of both genome and transcriptome sequencing data enabled the identification of allele-specific expression. Such an analysis was performed for genes from the sex-determining region (SDR).

High-Quality Genome Assembly of f. sp. .

In the present work, a highly pathogenic isolate of f. sp. , which is the most harmful pathogen affecting flax ( L.