Pioneering Activity of the C-Terminal Domain of EBF1 Shapes the Chromatin Landscape for B Cell Programming.

Lymphopoiesis requires the activation of lineage-specific genes embedded in naive, inaccessible chromatin or in primed, accessible chromatin. The mechanisms responsible for de novo gain of chromatin accessibility, known as "pioneer" function, remain poorly defined. Here, we showed that the EBF1 C-terminal domain (CTD) is required for the regulation of a specific gene set involved in B cell fate decision and differentiation, independently of activation and repression functions.

Transcription factor EBF1 is essential for the maintenance of B cell identity and prevention of alternative fates in committed cells.

The transcription factors EBF1 and Pax5 have been linked to activation of the B cell lineage program and irreversible loss of alternative lineage potential (commitment), respectively. Here we conditionally deleted Ebf1 in committed pro-B cells after transfer into alymphoid mice. We found that those cells converted into innate lymphoid cells (ILCs) and T cells with variable-diversity-joining (VDJ) rearrangements of loci encoding both B cell and T cell antigen receptors.

Validity and reliability of a Turkish version of the body shape questionnaire among female high school students: preliminary examination.

The aim of this study is to assess the validity and reliability of a Turkish version of the Body Shape Questionnaire (BSQ) among young people. The BSQ was initially administrated to female high school students (N = 665) and administered a second time to a subset of subjects (N = 144). The subjects also completed the Eating Attitudes Test, the Body Image Satisfaction Questionnaire (a dieting questionnaire) and the Hopkins Symptom Checklist (SCL-90), and were weighed, and their body mass indices were calculated.