Publications by authors named "Duxia Yang"
Article Synopsis
- The study discusses how esterase/lipase-catalyzed hydrolysis of d, l-menthyl esters is a promising method for producing l-menthol, a key flavoring chemical, though current biocatalysts lack sufficient activity and selectivity.
- Researchers cloned and engineered a highly active -nitrobenzyl esterase (pnbA-BS) to improve l-enantioselectivity by substituting Ala400 with Pro, significantly increasing the l-enantioselectivity value from 1.0 to 466.6.
- The study also incorporated a green approach by eliminating organic solvents and employing a constant feeding method, resulting in a 48.9% conversion of d, l-menth
Article Synopsis
- The study highlights the use of old yellow enzymes, particularly OYE3, for converting (/)-citral into enantio-pure ()-citronellal through asymmetric reduction.
- By modifying OYE3 using semi-rational design, specific variants (W116A and S296F) were created to improve enantioselectivity, effectively discriminating between different forms of citral.
- The best-performing variant, S296F/W116G, enabled a novel cascade reaction leading to the synthesis of ()-citronellal while preserving ()-citral, demonstrating an efficient method for producing enantiomerically pure compounds.
