In Vivo acrylamide exposure may cause severe toxicity to mouse oocytes through its metabolite glycidamide.

High acrylamide (ACR) content in heat-processed carbohydrate-rich foods, as well as roasted products such as coffee, almonds etc., has been found to be as a risk factor for carcinogenicity and genotoxicity by The World Health Organization. Glycidamide (GLY), the epoxide metabolite of ACR, is processed by the cytochrome P-450 enzyme system and has also been found to be a genotoxic agent.

Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?

Purpose: The present study aimed to evaluate whether combining the magnetic-activated cell sorting (MACS) with density-gradient (DG) or swim-up (SU) sperm separation techniques can improve sperm selection to obtain higher quality spermatozoa.

Methods: Two commonly used sperm selection techniques, SU and DG, were compared to MACS combined with either SU or DG. Spermatozoa obtained from normozoospermic (n = 10) and oligozoospermic (n = 10) cases were grouped as SU, DG, SU+MACS, and DG+MACS followed by the analysis of sperm morphology, motility, DNA integrity, and the levels of Izumo-1 and PLCZ proteins.

Can dicoumarol be used as a gonad-safe anticancer agent: an in vitro and in vivo experimental study.

Study Hypothesis: Dicoumarol (DC) has potential for use as a gonad-safe anticancer agent.

Study Finding: DC altered cell proliferation, decreased viability and increased apoptosis in Vero and MCF-7 cell lines but did not show any toxic effect on mouse ovarian tissues and developing oocytes in vitro and in vivo.

What Is Known Already: DC suppresses cell proliferation and increases apoptosis in various cancer cells such as breast, urogenital and melanoma.