Labelling quality and chromosome morphology after low temperature FISH analysed by scanning far-field and near-field optical microscopy.

A non-enzymatic, low temperature fluorescence in situ hybridization (LTFISH) procedure was applied to metaphase spreads and interphase cell nuclei. In this context 'low temperature' means that the denaturation procedure of the chromosomal target DNA usually applied by heat treatment and chaotropic agents such as formamide was completely omitted so that the complete hybridization reaction took place at 37 degrees C. For LTFISH, the DNA probe had to be single-stranded, which was achieved by means of separate thermal denaturation of the DNA probe only.

Sex, perception of immediate stress, and response to coping resources inventory, emotional domain.

The purpose was to assess differences in mean scores of 23 men and 28 women who rated immediate stress low and 11 men and 34 women who rated immediate stress high. Analysis of variance of mean differences was significant for sex but not for immediate stress or their interaction on the Coping Resources Inventory, Emotional Domain.

Construct validity for self-acceptance and fear of negative evaluation.

For 55 students (13 men, 42 women) there was a significant inverse correlation for scores on the Self-acceptance Scale and scores on the Fear of Negative Evaluation Scale, thereby giving credence to the construct validity of both scales, that is, the more one accepts oneself, the less negative evaluation there is of oneself.

External validity of religiosity scales for a sample of southern students.

When mean scores on the Spiritual or Philosophical Domain of the Coping Resources Inventory for 96 university students of ages 20 to 47 years, were compared with means of the standardization sample, values fell outside the upper limit of the 99% confidence interval, suggesting the scale may not have external validity for a sample of students.

Relation of self-acceptance and acceptance of others.

A significant positive correlation of .33 was found for ratings on the Self-acceptance Scale and on the Acceptance of Others Scale for 13 undergraduate men and 45 women.

Relationship between colorectal cancer glutathione levels and patient survival: early results.

Purpose: Elevated glutathione is a cause of resistance to anticancer agents and x-rays. The purpose of this study was to determine the frequency and clinical significance of glutathione elevation in human colorectal cancer.

Methods: Glutathione levels were measured in 41 colon cancers, 24 rectal cancers, and corresponding normal tissues.

Do sex and perception of immediate stress affect optimism?

The purpose was to assess differences in mean optimism scores of men and women of low immediate stress (23 men, 28 women) and high immediate stress (11 men, 34 women). Using factorial analysis of variance, mean differences on the Coping Resources Inventory, Cognitive Domain, were not significant for sex, immediate stress, or their interaction.

Substance abuse and academic and career problems for three age groups of college students.

Scores on the Substance Abuse, the Academic Problems, and the Career Problems subscales of the College Adjustment Scales were compared for 30 college students in three age groups. No significant mean difference was found among age groups on any scale.

College adjustment and older students.

When mean scores on the College Adjustment Scale for 21 students, ages 31 to 51 years, were compared with means of the standardization sample, values fell well within the acceptable ranges of 42nd to 67th percentiles, suggesting the scale could be given older students.

Marriage and stress-coping among female college students.

By an independent t test, mean scores on the social domain of the Coping Resources Inventory for 18 single and for 18 married female students were not significantly different, suggesting similar involvement in social networks supportive during stress.

Longevity in age-heterogamous marriages.

A post facto analysis was conducted to study the relationship between longevity and age-heterogamous marriages with data from 776 cemetery records. A significant positive linear relationship was found between age difference and age at death of men. No significant relationship was found for women.

Fast-painting of human metaphase spreads using a chromosome-specific, repeat-depleted DNA library probe.

For chromosome painting, in situ suppression of repetitive DNA sequences has been well established. Such standard protocols usually require large amounts of Cot-I DNA. Recently, it has become possible to deplete repetitive DNA sequences from library probes by magnetic purification and PCR-assisted affinity chromatography.

Fast-FISH technique for rapid, simultaneous labeling of all human centromeres.

Fluorescence in situ hybridization (FISH) has become a powerful tool in chromosome analysis. This report describes the systematic optimization of the Fast-FISH technique for centromere labeling of human metaphase chromosomes for radiobiological dosimetry purposes. For the present study, the hybridization conditions and the efficiency of two commercially available alpha-satellite DNA probes were compared ("human chromosome 1 specific", Oncor, Gaithersburg, MD, vs.

Is sex of fetus associated with duration of labor in nulliparas?

In a post facto study to examine whether sex of fetus was related to duration of nulliparas' labor 30 nulliparous (had not given birth previously) women who delivered boys and 30 others who delivered girls were matched according to the criteria that they were nulliparous, received an epidural for analgesia during labor, and their labors were either induced or augmented. All delivered vaginally. Duration of labor was not statistically significantly related to sex of the fetus.

Children's self-esteem and their parents' education.

A randomly selected sample of 51 high school students from 1000, 21 boys and 30 girls, completed Forms A and AD of the Culture-free Self-esteem Inventory and a data sheet giving grade, gender, and both parents' education. No significant correlation was found between parents' education and children's scores on self-esteem but a correlation was .70 between forms.

Optimized Fast-FISH with alpha-satellite probes: acceleration by microwave activation.

It has been shown for several DNA probes that the recently introduced Fast-FISH (fluorescence in situ hybridization) technique is well suited for quantitative microscopy. For highly repetitive DNA probes the hybridization (renaturation) time and the number of subsequent washing steps were reduced considerably by omitting denaturing chemical agents (e.g.

Non-enzymatic, low temperature fluorescence in situ hybridization of human chromosomes with a repetitive alpha-satellite probe.

In all DNA-DNA in situ hybridization (ISH) procedures described so far in the literature, the production of single-stranded target DNA sequences plays a decisive role. This can be achieved either by enzymatic treatment at physiological temperatures or by the separation of double-stranded DNA sequences. Denaturation by heat and chemical agents (e.

Optimization of Fast-FISH for alpha-satellite DNA probes.

It has been shown for several highly repetitive DNA probes that the newly introduced Fast-FISH (fast-fluorescence in situ hybridization) technique is well suited for quantitative microscopy. The advantage of omitting denaturing chemical agents (e.g.

Children of divorce and its effect on their self-esteem.

The purpose was to investigate the temporal relationship of divorce with self-esteem of children and to assess differences in self-esteem, if any, between children of divorced families and children of intact families. The self-esteem of 60 children in Grade 9 and from divorced homes was measured using the Culture-free Self-esteem inventory. There was no significant positive correlation between the passage of time and higher self-esteem among these children.

Painting of human chromosome 8 in fifteen minutes.

The technique of chromosome-in-situ suppression (CISS)-hybridization (chromosome painting) has now been well established. However, all standard protocols so far require long renaturation times (typically 12 hours and more). Here, we describe a new, extremely fast protocol for chromosome painting using a commercially available, directly fluorescence labelled probe for chromosome 8.

Does self-concept depend on body image? A gender analysis.

The purpose of this study was twofold, to estimate positive correlations between scores on the Tennessee Self-concept Scale for body image and self-concept within each gender and to assess differences between genders on measures of these two concepts, thereby testing the hypothesis that women have a more diminished body image than men. The study included 36 subjects, 18 male and 18 female. Within each gender were significant and positive correlations between measures of body image and self-concept.

Optimization of fast-fluorescence in situ hybridization with repetitive alpha-satellite probes.

A rapid FISH (fluorescence in situ hybridization) technique (Fast-FISH) for quantitative microscopy has been recently introduced. For highly repetitive DNA probes the hybridization (renaturation) time and the number of necessary washing steps were reduced considerably by omitting formamide or equivalent denaturing chemical agents. Due to low stringency conditions major and minor binding sites of the probes used showed visible FISH signals well suited for quantitative image-microscopy.

Intratumor variability in prognostic indicators may be the cause of conflicting estimates of patient survival and response to therapy.

The DNA index, percentage of S-phase cells, proliferation fraction, and glutathione (GSH) content were determined at more than 1100 separate sites in 140 human tumors and 140 normal tissues. The study showed that the variability was so great from site to site within a tumor that there was only a 61% chance of identifying an aneuploid tumor clone (when present) if only a single site sample was analyzed for DNA content. Similar broad variability was observed in the percentage of S-phase cells, proliferation fraction, and glutathione content.

Rapid fluorescence in situ hybridization with repetitive DNA probes: quantification by digital image analysis.

Fluorescence in situ hybridization (FISH) has become an important tool not only in cytogenetic research but also in routine clinical chromosome diagnostics. Here, results of a quantification of fluorescence signals after in situ hybridization with repetitive DNA probes are reported using a non-enzymatic hybridization technique working with a buffer system not containing any formamide or equivalent chemical denaturing agents. Following simultaneous denaturation of both cells and DNA probes, the renaturation time was reduced to less than 30 min.