Microchip isotachophoresis for green and sustainable pharmaceutical quality control: Method validation and application to complex pharmaceutical formulations.

Universal microchip isotachophoresis (μITP) methods were developed for the determination of cationic and anionic macrocomponents (active pharmaceutical ingredients and counterions) in cardiovascular drugs marketed in salt form, amlodipine besylate and perindopril erbumine. The developed methods are characterized by low reagent and sample consumption, waste production and energy consumption, require only minimal sample preparation and provide fast analysis. The greenness of the proposed methods was assessed using AGREE.

Maternal and infant predictors of proinflammatory milk immune activity in Kilimanjaro, Tanzania.

Objectives: The immune system of milk (ISOM) creates a mother-infant immune axis that plays an important role in protecting infants against infectious disease (ID). Tradeoffs in the immune system suggest the potential for both protection and harm, so we conceive of two dimensions via which the ISOM impacts infants: promotion of protective activity and control of activity directed at benign targets. High variability in ISOM activity across mother-infant dyads suggests investment the ISOM may have evolved to be sensitive to maternal and/or infant characteristics.

Primary assessment of medicines for expected migrastatic potential with holographic incoherent quantitative phase imaging.

Solid tumor metastases cause most cancer-related deaths. The prevention of their occurrence misses suitable anti-metastases medicines newly labeled as migrastatics. The first indication of migrastatics potential is based on an inhibition of enhanced migration of tumor cell lines.

Does the immune system of milk increase activity for infants experiencing infectious disease episodes in Kilimanjaro, Tanzania?

Introduction: Multiple studies have reported that milk immune content increases for infants experiencing infectious disease (ID) episodes, suggesting that the immune system of milk (ISOM) offers enhanced protection when needed to combat ID.

Methods: To test the hypothesis that ISOM content and/or activity increases during an infant's ID episode, we characterized milk secretory immunoglobulin A (sIgA; a major ISOM constituent) and in vitro interleukin-6 (IL-6) responses to Salmonella enterica and Escherichia coli, as system-level biomarkers of ISOM activity, in a prospective study among 96 mother-infant dyads in Kilimanjaro, Tanzania.

Results: After control for covariates, no milk immune variables (sIgA, Coef: 0.

Tradeoffs in milk immunity affect infant infectious disease risk.

Background And Objectives: The human immune system has evolved to balance protection against infection with control of immune-mediated damage and tolerance of commensal microbes. Such tradeoffs between protection and harm almost certainly extend to the immune system of milk.

Methodology: Among breastfeeding mother-infant dyads in Kilimanjaro, Tanzania, we characterized proinflammatory milk immune responses to (an infectious agent) and (a benign target) as the increase in interleukin-6 after 24 h of incubation with each bacterium.

Development of Microchip Isotachophoresis Coupled with Ion Mobility Spectrometry and Evaluation of Its Potential for the Analysis of Food, Biological and Pharmaceutical Samples.

An online coupling of microchip isotachophoresis (µITP) with ion mobility spectrometry (IMS) using thermal evaporation interface is reported for the first time. This combination integrates preconcentration power of the µITP followed by unambiguous identification of trace compounds in complex samples by IMS. Short-chain carboxylic acids, chosen as model analytes, were first separated by the µITP in a discontinuous electrolyte system at pH 5-6, and subsequently evaporated at 130 °C during their transfer to the IMS analyzer.

Coherence gate manipulation for enhanced imaging through scattering media by non-ballistic light in partially coherent interferometric systems.

Coherence gating is typically exploited for imaging through disordered media by least-scattered (ballistic) light. Ballistic light-based approaches produce clear images only when the proportion of ballistic to multiply scattered (non-ballistic) light is relatively high. To overcome this limitation, we counterintuitively utilize the coherence gate to image by the non-ballistic light, enabling us to retrieve information missing in the ballistic image.

Functionality and Palatability of Yogurt Produced Using Beetroot Pomace Flour Granulated with Lactic Acid Bacteria.

Following the idea of sustainability in food production, a yogurt premix based on beetroot () pomace flour (BPF) was developed. BPF was granulated with lactose solution containing lactic acid bacteria (LAB) by a fluidized bed. Particle size increased ~30%.

[Results of surgical treatment for lumbar spine segmental instability].

Aim: To perform a comparative analysis of outcomes in patients with lumbar spine segmental instability who underwent surgical treatment using transforaminal lumbar interbody fusion (TLIF) and direct lateral interbody fusion (DLIF) techniques.

Material And Methods: The multicenter study involved 209 patients who underwent surgery for lumbar spine segmental instability. Long-term outcomes (up to 2 years) were studied in 134 patients: patients of the first group (98 patients) underwent traditional transforaminal lumbar interbody fusion (TLIF) and transpedicular stabilization of vertebral segments; patients of the second group (36 patients) underwent direct lateral interbody fusion (DLIF) in combination with transpedicular stabilization of the lumbar vertebral segments.

Cerebral malaria complicated with coma, multiorgan failure and psychosocial sequellae observed among non-immune travelers to Benin, Sudan and Madagascar.

Objective: Severe malaria represents less than 10% of all malaria cases and is associated with significant mortality. The aim of this case series was to review severe malaria cases in travelers within the last 10 years in Slovakia.

Methods: All cases of severe malaria in travelers reported within last 10 years from the Inpatient Department in Slovakia to the Slovak Tropical Institute (STI) are reviewed.

Point-of-care platelet function tests: detection of platelet inhibition induced by nonopioid analgesic drugs.

Detection of platelet inhibition is of clinical relevance in the preinterventional risk-benefit assessment in chronic low-back-pain patients scheduled for invasive pain therapy. We evaluated the sensitivity of various point-of-care platelet function tests for the detection of platelet inhibition induced by nonopioid analgesic drugs. After Institutional Review Board approval and informed consent, citrated whole blood from 40 patients with chronic unspecific low back pain was investigated before and 30 min after intravenous infusion of the study medication consisting of diclofenac 75 mg (plus orphenadrin 30 mg; Neodolpasse; Fresenius Kabi Austria GmbH, Austria), parecoxib 40 mg (Dynastat; Pharmacia Europe EEIG, UK), paracetamol 1 g (Perfalgan; Bieffe Medital S.

Garlic at dietary doses does not impair platelet function.

Background: In vitro studies suggest that various bioactive constituents of Allium sativum (garlic) inhibit platelet function. The extent, however, to which dietary doses of garlic influence platelet function remains unknown. Therefore, we tested the effect of raw garlic on platelet function using point-of-care monitoring devices sensitive for cyclooxygenase I-inhibition and platelet adhesion.

Cerebral malaria in children in South Sudan: 8 years experience in 261 cases.

Two hundred-sixty-one (261) cases of cerebral malaria within last 8 years from 3 tropical clinics in South Sudan were analyzed. Coma was present at 79.8% and convulsions at 25.

Health risk of urban soils contaminated by heavy metals.

The paper presents the results of geochemical investigations carried out in the city of Prague. Czech Republic, between the years 1994-1997, by the Czech Geological Institute, National Institute of Public Health and Hygiene Stations of Prague. Exposure assessment for children as the most sensitive population, based on soil ingestion pathway, indicates that lead is the major pollutant of concern, especially in the central regions of the city.

Specificity of different anti-myosin heavy chain antibodies in bovine muscle.

Muscle fibre types play an important role in bovine meat tenderness, and conditions the speed of ageing. Two generations of myotubes appear during foetal life. Their differentiation results in several types of fibres differing in their contractile and metabolic properties, namely type I fibres and type II fibres (IIA and IIB).

Genetic variability of foetal bovine myoblasts in primary culture.

Selected strains of adult bovines and those which either have high muscle growth capacity or are double-muscled present particular characteristics of muscle fibres and collagen at slaughter that favour meat tenderness. For double-muscled bovines, it has been shown that these characteristics originated during foetal life. However, no studies have been done to determine the origin of muscle growth superiority in bovine with high muscle growth capacity compared to those with a low muscle growth capacity.

Electrophoretic separation of bovine muscle myosin heavy chain isoforms.

This study concerns the definition of the optimum conditions for separation of adult and developmental myosin heavy chain (MHC) isoforms in bovine muscle. The various techniques published do not result in good separation of the MHC in this species. The trials carried out concerned the concentration of acrylamide and N, N'-methylene-bis-acrylamide, and more particularly the concentration of Tris in the separating gel.

Classification of bovine muscle fibres by different histochemical techniques.

The classification of bovine muscle fibres is of particular interest for the food industry because meat tenderness depends in part on the proportion of the different types of fibres. It is, therefore, important to define reliable methods for classifying fibre types. There are several classification systems.