Determinants of multimorbidity among elderly population in maharashtra, India: Logistic regression analysis.

Background: Population aging is an emerging global trend. Because of decreasing fertility rates and improved healthcare, the lifespan of elderly population increased. Consequently, proportion of elderly population is increasing at an alarming rate.

Genetic polymorphisms of CYP1A, CYP1B, CYP2C and risk of cervical cancer among rural population of Maharashtra: Findings from a hospital-based case-control study.

Background: Last few decades, multiple studies all over the world revealed the association of genetic polymorphism in cytochrome P450 (CYP) genes with risk of developing different type of cancers, but contradictory outcomes were evidenced in case of cervical cancer (CC) risk. Therefore, the discrepancies in earlier reports influenced us to evaluate the association of CYP1A1*2A rs4646903, CYP1B1*3 rs1056836, CYP2C8*2 rs11572103, CYP2C9*2 rs1799853, CYP2C9*3 rs1057910, and CYP2C19*2 rs4244285 polymorphisms and CC susceptibility in the women of rural population of Maharashtra.

Materials And Methods: In this case-control study, genetic association of the polymorphisms in CYP genes was studied by using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method.

Superoxide Dismutase (rs2070424, rs4880, rs2536512) and Catalase (rs794316, rs1001179) SNPs and their Association with Breast Cancer Risk: Findings from a Hospital Based Case-Control Study.

Background: The antioxidant enzymes are important cellular components involved in detoxification of reactive oxygen species (ROS) and protect cells from ROS induced oxidative damage. Single nucleotide polymorphisms (SNPs) of antioxidant enzyme coding genes such as superoxide dismutase (SOD) and catalase (CAT) may alter the enzyme activity which can influence susceptibility towards carcinogenesis.  Therefore, the present study was planned to investigate possible SNPs of SOD (SOD1 (Cu,Zn-SOD), SOD2(Mn-SOD), SOD3(EC-SOD) and CAT genes and their possible association with breast cancer risk in rural Indian women.

Impact of Interaction between Single Nucleotide Polymorphism of XRCC1, XRCC2, XRCC3 with Tumor Suppressor Tp53 Gene Increases Risk of Breast Cancer: A Hospital Based Case-Control Study.

Background: At present very little information is available on combined effects of DNA repair genes with tumor suppressor gene polymorphisms and their association with cancer susceptibility. No such association studies have been carried out with breast cancer or any other cancer from India. Present study was conducted to study the combined effects of SNPs of XRCC1, XRCC2, XRCC3 with Arg72Pro and Arg249Ser SNPs of TP53 gene in risk of BC in rural parts of India.

Green Synthesis of Silver Nanoparticles using Linn LeafExtract and its Antioxidant, Antibacterial and Anticancer Potential.

Introduction: Nanomedicine has emerged as a revolutionary regimen for moderating communicable as well as non-communicable diseases.

Purpose: This study demonstrated the phytosynthesis of silver nanoparticles using leaf extract (MC-AgNPs) and their antioxidant, antibacterial and anticancer potential.

Materials And Methods: The Biosynthesis of MC-AgNPs was studied by spectroscopy and characterized by SEM, TEM, XRD and FTIR analysis.

Biogenic Nanoparticles: Synthesis, Characterization, and Biological Potential of Gold Nanoparticles Synthesized using Plant Extract.

Introduction: Recent advancements in biomedicine have revolutionized nanomedicine as a therapeutic moderator in the management of both infectious and noninfectious diseases.

Purpose: In the current study we demonstrated biosynthesis of gold nanoparticles using aqueous leaf extract of as a capping and reducing agent and evaluation of their antioxidant, antibacterial, and anticancer properties.

Methods: The biosynthesized LE-AuNPs were characterized by UV-Vis spectrophotometry, SEM, TEM, XRD, FTIR, DLS, and Zeta potential analysis.

Biogenic Synthesis of Silver Nanoparticles using (Decne): Assessment of their Antioxidant, Antimicrobial and Cytotoxic Activities.

Introduction: The emergence of novel nanobiomedicine has transformed the management of various infectious as well as non-infectious diseases., a medicinal plant, revealed the presence of active secondary metabolites and biological potentials.

Objective: The present study was aimed to demonstrate the biosynthesis of silver nanoparticles using leaf extract (LE-AgNPs) and their biological properties, such as antioxidant, antibacterial and anticancer potential.

CYP2D6 and CYP2E1 Gene Polymorphisms and their Association with Cervical Cancer Susceptibility: A Hospital Based Case-Control Study from South-Western Maharashtra.

Background: In last few years several studies all over the world discovered the genetic polymorphisms in different cytochrome P450 genes associated with risk of various cancers, but contradictory outcomes were evidenced in case of cervical cancer risk.  In this case-control study we aimed to see whether the polymorphism of CYP2D6 or CYP2E1 genes may or may not be associated with cervical cancer risk in women of rural Maharashtra.

Methods: In this case-control study, the association of CYP2D6 and CYP2E1 gene polymorphism with cervical cancer risk was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

Impact of Polymorphism in Base Excision Repair and Nucleotide Excision Repair Genes and Risk of Cervical Cancer: A Case-Control Study.

Background: Last few years, several studies all over the world revealed the association of DNA repair genes with risk of developing different type of cancers, but were ambiguous to support the evidences in case of cervical cancer risk. These differences in earlier studies directed us to study the association of polymorphisms of BER genes (XRCC1, hOGG1, XPC) and NER genes (XPC, XPD) with cervical cancer susceptibility in the women of rural population of Maharashtra.

Materials And Methods: The genetic polymorphism in BER and NER pathway genes was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using DNA isolated from intravenous blood samples of patients and normal controls.

Study to Assess Birth Preparedness and Complication Readiness to Promote Safe Motherhood among Women from a Rural Area of Western Maharashtra.

Background: Promotion of maternal health should be an integrated approach comprising adequate planning of pregnancy coupled with the awareness of the available maternal and child health services and its utilization.

Objectives: The aim of this study is to determine birth preparedness and complication readiness (BPACR) among antenatal and postnatal women and to assess the factors related to it.

Materials And Methods: This hospital-based cross-sectional study was conducted on 400 antenatal and postnatal women attending a tertiary care hospital of Karad.

Biogenic synthesis of gold nanoparticles using Argemone mexicana L. and their cytotoxic and genotoxic effects on human colon cancer cell line (HCT-15).

Background: Nanomedicine has evolved as precision medicine in novel therapeutic approach of cancer management. The present study investigated the efficacy of biogenic gold nanoparticles synthesized using Argemone mexicana L. aqueous extract (AM-AuNPs) against the human colon cancer cell line, HCT-15.

Gaps in facilities available at Community Health Centers/Rural Hospitals as per Indian public health standards - Study from Western Maharashtra.

Background: The launch of the National Rural Health Mission (NRHM) gives us the opportunity to review the functioning and bring up the Community Health Centers (CHC) services to the level of Indian Public Health Standards and thus improve the lives of citizens.

Objectives: Assessment of the gaps in the facilities available at Community health centers/Rural hospitals as per Indian Public health standards.

Methods: Facility based cross-sectional study was conducted in the Satara district of Maharashtra.

Menstrual hygiene among adolescent girls - A study from urban slum area.

Background: Adolescence has been recognized as a special period that requires specific attention as it marks the onset of menarche, an important milestone, and hence good hygienic practices during menstruation are crucial to maintain a healthy life.

Aims And Objectives: This study was planned to assess knowledge, beliefs, and source of information regarding menstruation, and also to assess hygiene among them.

Materials And Method: A cross-sectional study was carried out in urban slum area.

Evaluation of oxidative stress and anti-oxidant status in essential hypertension.

The present study was planned to access the role of oxidative stress and anti-oxidant status in essential hypertension. Malondialdehyde is one of the by-products of lipid peroxidase and so extent of lipid peroxidation was measured by estimating malondialdehyde levels, nitrite concentration was used as an index of nitric oxide synthesis and anti-oxidant status was measured in terms of total antioxidant capacity. One hundred and eighty cases with essential hypertension and 60 volunteers as healthy controls were selected for the study.

Differential trend of leprosy in rural and urban area of western Maharashtra.

Leprosy, major public health problem in Satara District with prevalence rate of 61/10000 population in 1990. With a view to eliminate Leprosy, Government of India launched National Leprosy Eradication Programme (NLEP) in Satara district during July 1990 to overcome the burden of Leprosy with Multi-Drug Treatment and subsequently Modified Leprosy Elimination Campaign and Block Leprosy Awareness Campaign. The objective of the study is to assess the differential trend of Leprosy in rural and urban area of Satara district.

A study of the outbreak of Chikungunya fever.

Background And Objectives: Chikungunya fever occurred in an epidemic form in the state of Maharashtra after a gap of about 32 years. Many cases with symptoms which were suggestive of Chikungunya fever were reported from the village Kasegaon, Dist Sangli, Maharashtra, India. Hence, this study was done to assess the magnitude of the outbreak and to identify the possible socio-environmental factors which are responsible for Chikungunya fever.

A Comparative Study of Clinical Utility of Spot Urine Samples with 24-h Urine Albumin Excretion for Screening of Microalbuminuria in Type 2 Diabetic Patients.

Unlabelled: Twenty-four hour urinary albumin excretion (UAE) is considered as gold standard method for albuminuria measurement, but collection of 24-h urine is inconvenient. The aim of present study was to evaluate whether albumin: creatinine ratio (ACR) and urinary albumin concentration (UAC) in different spot urine samples correlate or not with 24-h UAE for screening of microalbuminuria in type 2 diabetic patients. We collected first morning void (FMV), random urine sample (RUS) and 24-h urine, separately on consecutive days from 104 type 2 diabetic patients.

Evaluation of national leprosy eradication programme in Satara District, Maharashtra.

The aim of this study was to evaluate the impact of national leprosy eradication programme (NLEP) in Satara District. A retrospective record based study was conducted in the district leprosy control unit with evaluation indicators like prevalence rate (PR), new case detection rate (NCDR), percentage of cases released from treatment, proportion of female cases among new cases, proportion of multi bacillary (MB) cases among new cases, proportion of grade-2 disability among new cases and proportion of child cases among new cases and trend was analyzed for statistical significance by using Chi-square test. During study period from year March 2003-04 to March 2007-08, there was decrease in PR of leprosy from 2.

A study of some clinical variables in predicting outcome after acute myocardial infarction.

The univariate analysis of age, previous myocardial infarction, systolic blood pressure, diabetes, smoking, etc, accounted their association with increased mortality in acute myocardial infarction (AMI). Thus, the study was undertaken to determine the significance of some such variables among hospitalised AMI cases by applying univariate statistical techniques. Two years retrospective data of 208 hospitalised AMI cases was collected from patients' records.

Evaluation of reference intervals of serum lipid profile from healthy population in Western Maharashtra.

Fasting samples of 914 subjects from healthy population were analyzed for total cholesterol, triglyceride and three major fractions of lipoproteins i.e. high-density lipoprotein cholesterol, low lipoprotein cholesterol and very low-density lipoprotein cholesterol.

A sensitive and economical modified method for estimation of cerebrospinal fluid proteins.

Cerebrospinal fluid protein estimation is a routine diagnostic tool for various diseases. The proposed modified bromocresol green (BCG) method is applicable to automated as well as manual measurements. Protein concentrations and absorbance at 600 nm are linearly related throughout a wide range of concentrations from 10 mg % to 2000 mg %.

Differential erythrocyte agglutination pattern in normal and cancer patients with Synadenium grantii root (Hook f) lectin.

In the present study the property of lectin agglutination in blood on normal and different cancer patients has been observed. The purifiedSynadenium grantii root lectin was non blood group specific and its utility as a diagnostic tool in malignancy was studied. Hemagglutination (units/ml) of red blood cells of different types of cancer was compared with the normal control's red blood cells.