Histopathological and biomechanical evaluation of tenocyte seeded allografts on rat Achilles tendon regeneration.

Tendon injuries in humans as well as in animals' veterinary medicine are problematic because tendon has poor regenerative capacity and complete regeneration of the ruptured tendon is never achieved. In the last decade there has been an increasing need of treatment methods with different approaches. The aim of the current study was to improve the regeneration process of rat Achilles tendon with tenocyte seeded decellularized tendon matrices.

Persistent organochlorine pesticide residues in water and sediment samples from Lake Manyas, Turkey.

The extent of organochlorine pesticides (OCPs) contamination in Manyas Lake (Bird Lake) and its tributary rivers and streams which is situated near the South-eastern coasts of the Marmara Sea in Turkey was investigated. Residues of OCPs in surface water and sediment samples were analysed by gas chromatography equipped with electron capture detector. The total OCPs concentration (sigmaOCPs) in water and sediment samples ranged from 1.

Carvacrol partially reverses symptoms of diabetes in STZ-induced diabetic rats.

Little is known about the protective effects of carvacrol on the symptoms of streptozotocin induced diabetes in rats. Hence, this present study was designed to evaluate the protective effect of the strong antioxidant, carvacrol, on the symptoms of streptozotocin induced diabetes in rats. Carvacrol at the doses of 25 and 50 mg/kg body weight were orally administered to diabetic rats for a period of 7 days after the onset of diabetes.

Inhibiting inducible nitric oxide synthase with rutin reduces renal ischemia/reperfusion injury.

Background: Nitric oxide (NO) seems to play an important role during renal ischemia/reperfusion (I/R) injury. We investigated whether rutin inhibits inducible nitric oxide synthase (iNOS) and reduces 3-nitrotyrosine (3-NT) formation in the kidneys of rats during I/R.

Methods: Wistar albino rats were nephrectomized unilaterally and, 2 weeks later, subjected to 45 minutes of left renal pedicle occlusion followed by 3 hours of reperfusion.

Gene expression of tendon collagens and tenocyte markers in long-term monolayer and high-density cultures of rat tenocytes.

As a result of repeated movement, tendons are functionally open to traumas. According to this situation, tenocytes have already been used for tissue engineering therapies. It has been reported that long-term monolayer (ML) culture of tenocytes may lead to a phenotypic drift within passages.

Evaluation of spatial and temporal changes in biomarker responses in the common carp (Cyprinus carpio L.) for biomonitoring the Meriç Delta, Turkey.

The aim of this study is to examine the activity of several biomarkers in carp, Cyprinus carpio L., to determine their response to xenobiotics, such as organochlorine pesticides (OCPs), in the Meriç Delta. Fish were collected from contaminated sites and from areas regarded as relatively less contaminated in four sampling periods.

Influence of vitamin C on bisphenol A, nonylphenol and octylphenol induced oxidative damages in liver of male rats.

The purpose of this study was to investigate whether bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP) induce oxidative stress in the liver of male rats and co-administration of vitamin C can prevent any possible oxidative stress. Wistar male rats were divided into seven groups (vehicle, BPA, NP, OP, BPA+C, NP+C, OP+C). BPA, OP and NP groups (25 mg kg(-1)day(-1)) were administered orally to rats three times a week for 50 days.

The effects of maternal exposure to food additive E341 (tricalcium phosphate) on foetal development of rats.

E341 (tricalcium phosphate) (TCP) is commonly used as a food additive and also as a nutritional supplement. To evaluate the possible developmental effects, female Wistar rats were treated with E341 (TCP) by oral gavage during pregnancy. There were three groups of each containing five rats.

Vitamin C coadministration augments bisphenol A, nonylphenol, and octylphenol induced oxidative damage on kidney of rats.

The aim of this study was to investigate whether bisphenol A (BPA), nonylphenol (NP), and octylphenol (OP) induce oxidative stress on the kidney tissue of male rats and whether coadministration of vitamin C, an antioxidant, can prevent any possible oxidative stress. The Wistar male rats were divided into seven groups, including control, BPA, NP, OP, BPA+C, NP + C, OP +C. BPA, NP, and OP (25 mg/kg/day) was administered alone; vitamin C (60 mg/kg/day) was administered along with BPA, OP, and NP to the rats for 50 days.

Pro-oxidant effect of vitamin C coadministration with bisphenol A, nonylphenol, and octylphenol on the reproductive tract of male rats.

This study was performed to investigate whether bisphenol A (BPA), nonylphenol (NP), and octylphenol (OP) induce oxidative stress on the reproductive tract of male rats and if coadministration of vitamin C can prevent any possible oxidative stress. Wistar male rats were divided into seven groups as control (vehicle; olive oil), BPA, NP, OP, BPA+C, NP+C, and OP+C. BPA, OP, and NP groups (25 mg/kg/day) were administered orally to rats three times a week for 45 days.

Protective effect of rutin on the ischemia/reperfusion induced damage in rat kidney.

Reactive oxygen species (ROS) are suggested to participate in ischemia/reperfusion (I/R) injury in the kidney. This study was designed to investigate the effect of rutin, a bioflavonoid, in I/R induced renal injury. Wistar albino rats were unilaterally nephrectomized, and 2 wk later they were subjected to 45 min of left renal pedicle occlusion followed by 3h of reperfusion.

Characterization of type I, III and V collagens in high-density cultured tenocytes by triple-immunofluorescence technique.

The purpose of this study is to examine the intracellular distribution of collagen types I, III and V in tenocytes using triple-label immunofluorescence staining technique in high-density tenocyte culture on Filter Well Inserts (FWI). The tenocytes were incubated for 4 weeks under monolayer conditions and for 3 weeks on FWI. At the end of the third week of high-density culture, we observed tenocyte aggregation followed by macromass cluster formation.

The protective effects of ascorbic acid against renal ischemia-reperfusion injury in male rats.

There is increasing evidence to suggest that toxic oxygen radicals play an essential role in the pathogenesis of ischemia/reperfusion (I/R) injury in the kidney. This study was designed to investigate the effects of ascorbic acid (AA) in I/R-induced renal injury in rats. Thirty two male Sprague-Dawley rats were divided equally into four groups: group 1 (control; dissection of the right renal pedicle without nephrectomy), group 2 (sham operated; unilateral nephrectomy), group 3 (I/R; unilateral nephrectomy + I/R); and group 4 (AA+I/R; unilateral nephrectomy and I/R treated with ascorbic acid, 250mg kg(-1) i.

Anzer honey prevents N-ethylmaleimide-induced liver damage in rats.

N-ethylmaleimide (NEM) is a sulphydryl blocker which impairs the sulphydryl dependent antioxidant system (mainly glutathione) in the body by alkylating endogenous sulphydryls. This study was designed to investigate the effects of Anzer honey on NEM-induced liver injury in rats. Thirty female Wistar albino rats were divided equally into three groups.

The effect of vitamin C on bisphenol A, nonylphenol and octylphenol induced brain damages of male rats.

Bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP) are endocrine-disrupting chemicals that has been shown to exert both toxic and estrogenic effects on mammalian cells. The aim of this study was to investigate if BPA, NP and OP induce oxidative stress on the brain tissue of male rats and if co-administration of vitamin C, an antioxidant, can prevent any possible oxidative stress. The male rats were divided into seven groups as control (vehicle), BPA, NP, OP, BPA+C, NP+C, OP+C.

The effect of sub-acute and sub-chronic exposure of rats to the glyphosate-based herbicide Roundup.

Roundup is a glyphosate-based herbicide that includes 78.5% glyphosate and surfactant at lower toxic concentrations. Glyphosate is an organophosphorated non-selective agrochemical widely used in many countries including Turkey and acts after the sprout in a systemic way.

The effect of Trifolium, Raphanus, and Cistus pollen grains on some blood parameters and mesentery mast cells.

Three kinds of pollen taxa belonging to 3 families (Fabaceae--Trifolium spp., Brassicaceae--Raphanus spp. and Cistaceae--Cistus spp.

Protective effect of Anzer honey against ethanol-induced increased vascular permeability in the rat stomach.

The purpose of this study was to determine the protective effect of Anzer honey on ethanol-induced increased vascular permeability in rats. Evan's Blue (EB) dye, administered intracardiacly and extravasation of EB into the stomach, served as an indicator of vascular permeability following exposure to alcohol. Ethanol was given orally to the ethanol group for 90 days, and N-etylmaleimide (NEM) was given subcutaneously to the NEM group, and we observed increased extravasation of EB in the stomach in both groups.