[Effects of Corbicula fluminea bioturbation on the community composition and abundance of ammonia-oxidizing archaea and bacteria in surface sediments].

To better understand the effects of Corbicula fluminea bioturbation on the ammonia-oxidizing microorganisms in the surface sediment, sediment-water microcosms with different densities of Corbicula fluminea were constructed. Clone libraries and real-time qPCR were applied to analyze the community composition and abundance of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in the surface sediments. The results obtained indicated that the bioturbation of Corbicula fluminea accelerated the release of nitrogen from the surface sediment.

[Distribution and diversity of Legionella spp. in Lake Taihu in the winter].

The Legionella spp. are ubiquitous in aquatic environment and could cause certain risks on human health. In order to investigate the distribution and diversity of Legionella spp.

[Damage analyse of X-ray radiated cellobiohydrolase II molecule from Trichoderma viride].

After X-ray treatment at 1.82 keV and 40 mA and 4 hours, the cellobiohydrolase II (CBH II) aqueous solution of Trichoderma viride was analysed, and the damage state of the irradiated molecule was detected using some cysteine residue relative parameters in Raman spectroscopic methods. The results show that S-H stretch modes of CBH II exhibited some shift, which means that the hydrogen proton donor state of sulfhydryl groups was stronger and weaker, respectively.

[Hydrogen-bond state analysis of cellobiohydrolase I molecule from Trichoderma viride].

Hydrogen-bond states on cellobiohydrolase II (CBH II) of Trichoderma viride were analysed by laser Raman spectroscopic method used to reveal molecular normal modes of vibration character. The results indicated that hydrogen-bond ability of carbonyl oxygen-atom of amide I was raised in two types of aqueous solution samples (6.0 and 8.

Improved stability and yield of Fv targeted superantigen by introducing both linker and disulfide bond into the targeting moiety.

Bacterial superantigens (SAg) are the most potent activators of human T lymphocytes and recombinant immunotoxin using bacterial SAg shows promising clinical values. To engineer superantigen for immunotherapy of hepatocellular carcinoma, we genetically fused the superantigen staphylococcus enterotoxin A (SEA(D(227)A)) to the single-chain disulfide-stabilized Fv (scdsFv) of anti-hepatoma monoclonal antibody HAb25 through a short peptide GGGSGGS. We expressed this recombinant protein in Escherichia coli and extract it from inclusion bodies.

[Expression, purification, and characterization of single-chain disulfide-bond Fv (ScdsFv) antibody fused with targeted superantigen SEA (D227A)].

Aim: To express, purify, and characterize scdsFv antibody fused with superantigen SEA(D227A).

Methods: The expression plasmid of scdsFv-SEA(D227A) was constructed by standard molecular cloning procedures. The recombinant protein was induced to express in E.