Effects of MicroRNA-10b on lung cancer cell proliferation and invasive metastasis and the underlying mechanism.

Objective: To study the influence of MicroRNA-10b on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.

Methods: Lipofectamine MicroRNA-10b eukaryotic expression plasmid was transfected into 95-C. The experiment group was divided into blank control group, empty vector transfected group and MicroRNA-10b transfected group.

Inducible Expression of bcl-2 Antisense RNA Promotes Apoptosis of Human Gastric Cancer Cell Line.

A lot of molecular biological techniques was used to observe the effect of bcl-2 antisence RNA on human gastric cancer cell line SGC7901 and to detect the inducibility and promotion of MT-II promoter. The results showed that MT-II promoter could be activated by the induction with 160 &mgr;mol/L Zn(2+) and the expression of Bcl-2 plays an important role in apoptosis of SGC7901 cells.