Two new megastigmane glycosides from the leaves of and their anti-inflammatory activities.

Two new megastigmane glycosides, (6 ,7,9)-3-oxo--ionyl-9-O--L-rhamnopyranosyl-(1''→4')--D-glucopyranoside () and (6 ,7,9)-3-oxo--ionyl-9-O--D-glucopyranosyl-(1''→6')--D-glucopyranoside (), together with six known analogues (-) were isolated from the leaves of . The structures of all metabolites were determined by comprehensive analysis of NMR and MS spectroscopic data as well as by comparison with those of previously reported. The anti-inflammatory activity of all isolates was evaluated using a lipopolysaccharide (LPS)-induced RAW264.

New Caffeoylquinic Acid Derivatives and Flavanone Glycoside from the Flowers of and Their Bioactivities.

The flower is widely used in China and Japan as a food, beverage, and medicine for many diseases. In our work, two new caffeoylquinic acid derivatives (), a new flavanone glycoside (), and six reported flavanones (⁻) were isolated and identified from the flowers of . The chemical structures of all isolates were elucidated by the analysis of comprehensive spectroscopic data as well as by comparison with previously reported data.

A new family-3 glycoside hydrolase from Penicillium oxalicum BL 3005 catalyzing tyrosol glucosylation to form salidroside.

A glycoside hydrolase from Penicillium oxalicum BL 3005 was purified to apparent homogeneity. Its molecular mass was estimated to be 90 kDa by SDS-PAGE. The enzyme was identified to be a new member of family-3 by peptide sequence.

Bioconversion of lutein to form aroma compounds by Pantoea dispersa.

Objectives: To investigate the conversion of lutein, a carotenoid, to aroma compounds by Pantoea dispersa Y08, a lutein-degrading bacterium isolated from marigold flower residue. Bioconversion conditions, including substrate concentration, applied co-solvent and reaction time, were optimized.

Results: A maximum biodegradation yield of 80 % for lutein at 10 g/l was achieved.

Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.

The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C. A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined.

Antioxidant activity potential of Virginia (flue-cured) tobacco flower polysaccharide fractions obtained by ultrasound-assisted extraction.

Ultrasound-assisted extraction was employed to extract polysaccharide from Virginia (flue-cured) tobacco flowers. The orthogonal matrix method (L9(3)(4)) was used to determine the optimal extraction conditions as to ultrasound power, extraction time, ratio of solvent to solid, and extraction temperature at 300 W, 4 min, 35 (mL/g), and 70 °C respectively. The crude extract was successively purified by chromatography, yielding two major polysaccharide fractions, termed Fr-I and Fr-II.

Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster.

We have expressed the pqqABCDE gene cluster from Gluconobacter oxydans, which is involved in pyrroloquinoline quinone (PQQ) biosynthesis, in Escherichia coli, resulting in PQQ accumulation in the medium. Since the gene cluster does not include the tldD gene needed for PQQ production, this result suggests that the E. coli tldD gene, which shows high homology to the G.

4,4'-(Anthracene-9,10-di-yl)dibenzoic acid dimethyl-formamide disolvate.

In the title compound, C(28)H(18)O(4)·2C(3)H(7)NO, the dihedral angle between the benzene rings and the anthracene system is 74.05 (12)°. A crystallographic inversion centre is located in the middle of the anthracene unit.

4-(2,5-Dihexyl-oxyphen-yl)benzoic acid.

In the title compound, C(25)H(34)O(4), one n-hexyl chain of the hex-yloxy group adopts a fully extended all-trans conformation, and the other n-hexyl chain displays disorder with site occupancies of 0.470 (3) and 0.530 (3).